FlyBase Allele Report: Dmel\crb[HMS01842]

General Information

Symbol

Dmel\crb^HMS01842

Species

D. melanogaster

Name

FlyBase ID

FBal0278959

Feature type

allele

Associated gene

Dmel\crb

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01842}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01842}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01842

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | second instar larval stage, with Scer\GAL4^btl.PS

(Skouloudaki et al., 2019)

abnormal cell shape | second instar larval stage, with Scer\GAL4^da.G32

(Skouloudaki et al., 2019)

abnormal mitotic cell cycle | embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(Yeom et al., 2015)

Phenotype Manifest In

embryonic/larval tracheal section | second instar larval stage, with Scer\GAL4^btl.PS

(Skouloudaki et al., 2019)

embryonic/larval tracheal section | second instar larval stage, with Scer\GAL4^da.G32

(Skouloudaki et al., 2019)

larval tracheocyte | second instar larval stage, with Scer\GAL4^btl.PS

(Skouloudaki et al., 2019)

larval tracheocyte | second instar larval stage, with Scer\GAL4^da.G32

(Skouloudaki et al., 2019)

Detailed Description

Statement

Reference

Second instar larvae expressing crb^HMS01842 under the control of either Scer\GAL4^da.G32 or Scer\GAL4^btl.PS show twisted tracheal tubes and lack of gas filling; Scer\GAL4^da.G32-driven expression leads to reduced apical surfaces of tracheal tube cells, despite of no obvious defects in apico-basal polarity. Scer\GAL4^btl.PS-driven expression leads to significantly smaller Vps35-positive vesicles in larval tracheal cells.

(Skouloudaki et al., 2019)

Embryos derived from females expressing crb^HMS01842 in the germline under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} show severe chromosome defects like chromosome bridges and bent chromosomes. They also show defects in spindle microtubules such as monopolar spindles and fusion with one or more neighbouring spindles. Anaphase chromosomes show high levels of PH3 staining in all chromosomal regions compared with wild type.

(Yeom et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, crb^HMS01842 has abnormal mitotic cell cycle | maternal effect | embryonic stage phenotype, suppressible | partially by galla-1^UAS.cYa/Scer\GAL4^{VP16.mat.αTub67C}

(Yeom et al., 2015)

Suppressor of

Statement

Reference

crb^HMS01842/Scer\GAL4^btl.PS is a suppressor | partially of abnormal size | second instar larval stage phenotype of apn¹

(Skouloudaki et al., 2019)

Phenotype Manifest In

Suppressor of

Statement

Reference

crb^HMS01842/Scer\GAL4^btl.PS is a suppressor | partially of vesicle | second instar larval stage phenotype of apn¹

(Skouloudaki et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of galla-1^Scer\UAS.cYa partially suppresses the chromosome segregation defects seen in embryos derived from mothers expressing crb^HMS01842 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.

(Yeom et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38373

y¹ v¹; P{TRiP.HMS01842}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

crb^HMS01842

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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