Expression of Apoltp^{dsRNA.Scer\UAS.II} in the fat body under the control of Scer\GAL4^Adh.PF (initiated by heat shock during the early larval stages) results in dramatic enlargement of the neutral fat droplets in the larval posterior midgut.

Expression of Apoltp^{dsRNA.Scer\UAS.II} under the control of Scer\GAL4^Rfabg.PB reduces the larval hemolymph levels of medium-chain diacylglycerol (DAG) (DAG26 and DAG28) and sterols by about 70%. In contrast, levels of PE, the major polar Lpp lipid, are not changed. The amounts of several minor lipid classes (phosphatidylcholine (PC), triacylglycerol (TAG), sphingolipids) increase slightly.

In larval guts expressing Apoltp^{dsRNA.Scer\UAS.II} under the control of Scer\GAL4^Adh.PF the levels of medium-chain diacylglycerol (DAG) increase 5-8 fold with respect to polar lipids. Long chain DAG increases moderately, and contributes less to the total elevation in gut DAG. Loss of Apoltp also leads to a strong increase in triacylglycerol (TAG) in the gut, with medium-chain TAG the most strongly elevated. An increase in TAG and DAG is seen in both lipid-fed and lipid-starved animals.

The fat bodies of larvae expressing Apoltp^{dsRNA.Scer\UAS.II} under the control of Scer\GAL4^Adh.PF contain much less sterol than those of wild type flies. Fat bodies also contain much less medium-chain diacylglycerol (DAG). Fat body triacylglycerol (TAG) storage is reduced by 30%, but the morphology of neutral lipid droplets is not obviously altered.

Expression of Apoltp^{dsRNA.Scer\UAS.II} under the control of Scer\GAL4^Adh.PF specifically depletes levels of medium chain diacylglycerol (DAG) in the wing disc. Long-chain DAG levels are unaffected. Total triacylglycerol (TAG) levels are reduced by 40% and the size and number of neutral lipid droplets are modestly reduced. The reduction in medium-chain TAG levels is more severe than the reduction in long-chain species. Long-chain PE levels are also unaffected.

Expression of Apoltp^{dsRNA.Scer\UAS.II} in the fat body under the control of Scer\GAL4^Adh.PF has no effect on neutral lipid levels in the larval brain.