FlyBase Allele Report: Scer\GAL4[apolpp.PB]

General Information

Symbol

Scer\GAL4^apolpp.PB

Species

S. cerevisiae

Name

Retinoid- and fatty acid-binding glycoprotein promoter construct of Brankatschk

FlyBase ID

FBal0247186

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Lpp-GAL4.B}

Also Known As

Lpp-GAL4, LppGal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Brankatschk and Eaton, 2010)

Progenitor genotype

Carried in construct

P{Lpp-GAL4.B}

Cytology

Description

A 5.5kb apolpp promoter fragment is fused upstream of sequence encoding a GAL4 driver. A 3kb 3'UTR fragment of apolpp is fused downstream of the GAL4 coding sequence.

(Brankatschk and Eaton, 2010)

Allele components

Component

Use(s)

Regulatory region(s)

apolpp

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14

yolk nucleus

(Palm et al., 2012)

larval stage

embryonic/larval fat body

(Van De Bor et al., 2015, Palm et al., 2012, Brankatschk and Eaton, 2010)

adult stage

adult fat body

(Van De Bor et al., 2015)

Additional Information

Statement

Reference

Drives expression in larval and adult fat bodies.

(FlyBase Curators, 2017)

ScerGAL4^apolpp.PB drives expression in yolk cells in embryos, and in fat bodies in larvae.

(Palm et al., 2012)

Marker for

Reflects expression of

apolpp

(Bloomington Drosophila Stock Center, 2017, Palm et al., 2012, Brankatschk and Eaton, 2010)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | adult stage, with Fbp1^GD5143

(Valzania et al., 2024)

abnormal developmental rate, with Fbp1^GD5143

(Valzania et al., 2024)

abnormal developmental rate, with Mtp^GD5414

(Baer et al., 2012)

abnormal developmental rate | larval stage, with Ctns^UAS.Tag:HA

(Jouandin et al., 2022)

abnormal developmental rate | larval stage | nutrition conditional, with Ctns^UAS.Tag:HA

(Jouandin et al., 2022)

abnormal immune response | adult stage, with cGlr2^UAS.cHa

(Ai et al., 2024)

abnormal mitotic cell cycle | larval stage, with apolpp^RNAi.UAS.cUa

(Brankatschk and Eaton, 2010)

abnormal neuroanatomy | second instar larval stage, with apolpp^RNAi.UAS.cUa

(Brankatschk and Eaton, 2010)

abnormal size, with Fbp1^GD5143

(Valzania et al., 2024)

abnormal starvation stress response | adult stage, with Fbp1^GD5143

(Valzania et al., 2024)

abnormal starvation stress response | adult stage, with Ire1^GD3071

(Zhao et al., 2021)

abnormal starvation stress response | adult stage, with Ire1^HMS03003

(Zhao et al., 2021)

decreased rate of adult locomotory behavior | larval stage, with Fbp1^GD5143

(Valzania et al., 2024)

decreased rate of cell adhesion | third instar larval stage, with NimB5^KK110850

(Ramond et al., 2020)

decreased size, with Pex11g^UAS.Tag:FLAG

(Liu and He, 2024)

decreased size | adult stage | progressive, with Arlr^GD6480

(Sun et al., 2023)

decreased size | adult stage | progressive, with Arlr^HMJ23623

(Sun et al., 2023)

decreased size | P-stage, with Ilp6^KK111727

(El Marzkioui et al., 2025)

female semi-sterile, with Fbp1^GD5143

(Valzania et al., 2024)

increased body size | larval stage, with Fbp1^GD5143

(Valzania et al., 2024)

increased body size | larval stage, with ITP^UAS.F

(Valzania et al., 2024)

increased body size | P-stage, with Fbp1^GD5143

(Valzania et al., 2024)

increased cell number | larval stage, with NimB5^HMC02406

(Ramond et al., 2020)

increased cell number | larval stage, with NimB5^KK110850

(Ramond et al., 2020)

increased mortality | adult stage, with Ire1^GD3071

(Zhao et al., 2021)

increased mortality | adult stage, with Ire1^HMS03003

(Zhao et al., 2021)

increased size | larval stage, with ITP^UAS.F

(Valzania et al., 2024)

lethal - all die before end of second instar larval stage, with apolpp^RNAi.UAS.cUa

(Brankatschk and Eaton, 2010)

majority die during pupal stage, with Dp^GD4444

(Zappia et al., 2021)

male semi-fertile, with Fbp1^GD5143

(Valzania et al., 2024)

melanotic mass phenotype | adult stage, with Hayan^UAS.cNa

(Nakano et al., 2023)

partially lethal, with Fbp1^GD5143

(Valzania et al., 2024)

partially lethal - majority die, with Dp^GD4444

(Zappia et al., 2021)

partially lethal - majority live | nutrition conditional, with NimB5^HMC02406

(Ramond et al., 2020)

partially lethal - majority live | nutrition conditional, with NimB5^KK110850

(Ramond et al., 2020)

short lived, with Arlr^GD6480

(Sun et al., 2023)

short lived, with Arlr^HMJ23623

(Sun et al., 2023)

short lived, with Fbp1^GD5143

(Valzania et al., 2024)

some die during pharate adult stage, with Dp^GD4444

(Zappia et al., 2021)

some die during second instar larval stage, with apolpp^RNAi.UAS.cUa

(Brankatschk and Eaton, 2010)

visible | adult stage, with Hayan^UAS.cNa

(Nakano et al., 2023)

Phenotype Manifest In

adult abdomen, with Fbp1^GD5143

(Valzania et al., 2024)

adult hemolymph, with Fbp1^GD5143

(Valzania et al., 2024)

embryonic/larval hemocyte | increased number | larval stage, with NimB5^HMC02406

(Ramond et al., 2020)

embryonic/larval hemocyte | increased number | larval stage, with NimB5^KK110850

(Ramond et al., 2020)

embryonic/larval hemolymph | larval stage, with Fbp1^GD5143

(Valzania et al., 2024)

embryonic/larval hemolymph | larval stage, with ITP^UAS.F

(Valzania et al., 2024)

embryonic/larval hemolymph | third instar larval stage, with apolpp^{RNAi.ApoLI.UAS}

(Palm et al., 2012)

embryonic/larval hemolymph | third instar larval stage, with Apoltp^RNAi.UAS.II

(Palm et al., 2012)

embryonic/larval optic lobe | second instar larval stage, with apolpp^RNAi.UAS.cUa

(Brankatschk and Eaton, 2010)

endoplasmic reticulum exit site | larval stage, with Tango1^NIG.11098R

(Ríos-Barrera et al., 2017)

fat body, with Tango1^NIG.11098R

(Ríos-Barrera et al., 2017)

fat cell | adult stage, with Grasp65^SH08450

(Rajan et al., 2017)

fat cell | adult stage, with Grasp65^SH08451

(Rajan et al., 2017)

lipid droplet, with Pex11g^UAS.Tag:FLAG

(Liu and He, 2024)

lipid droplet | adult stage, with Arlr^GD6480

(Sun et al., 2023)

lipid droplet | adult stage, with Arlr^HMJ23623

(Sun et al., 2023)

muscle cell of ventral longitudinal muscle 3 | third instar larval stage, with Dp^GD4444

(Zappia et al., 2021)

neuropil | second instar larval stage, with apolpp^RNAi.UAS.cUa

(Brankatschk and Eaton, 2010)

nucleus | increased number | third instar larval stage, with Dp^GD4444

(Zappia et al., 2021)

sarcomere, with Fbp1^GD5143

(Valzania et al., 2024)

wing, with Fbp1^GD5143

(Valzania et al., 2024)

wing | P-stage, with Ilp6^KK111727

(El Marzkioui et al., 2025)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^apolpp.PB/Def^KK111656 is an enhancer of neoplasia | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Def^SK3 is an enhancer of neoplasia | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB/imd^KK109863 is an enhancer of neoplasia | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB/Myd88^GD9716 is an enhancer of neoplasia | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, imd^KK109863, Def^UAS.cTa is an enhancer of neoplasia | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Suppressor of

Statement

Reference

Scer\GAL4^apolpp.PB/Def^KK111656 is a suppressor of increased cell death | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Def^SK3 is a suppressor of increased cell death | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB/imd^KK109863 is a suppressor of increased cell death | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB/Myd88^GD9716 is a suppressor of increased cell death | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, imd^KK109863, Def^UAS.cTa is a suppressor | partially of increased cell death | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Myd88^GD9716 is a suppressor | partially of increased cell death | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

NOT Suppressor of

Statement

Reference

Scer\GAL4^apolpp.PB/Hsap\UBA5^UAS.cBa is a non-suppressor of lethal - all die during embryonic stage | male phenotype of Uba5^KO

(Pan et al., 2023)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^apolpp.PB, Tango1^NIG.11098R has fat body phenotype, non-suppressible by Scer\GAL4^apolpp.PB/vkg^NIG.16858R

(Ríos-Barrera et al., 2017)

Enhancer of

Statement

Reference

Scer\GAL4^apolpp.PB/Def^KK111656 is an enhancer of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Def^SK3 is an enhancer of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB/imd^KK109863 is an enhancer of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB/Myd88^GD9716 is an enhancer of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, imd^KK109863, Def^UAS.cTa is an enhancer of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Suppressor of

Statement

Reference

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Def^SK3 is a suppressor of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB/imd^KK109863 is a suppressor of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB/Myd88^GD9716 is a suppressor of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, imd^KK109863, Def^UAS.cTa is a suppressor | partially of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Myd88^GD9716 is a suppressor of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB/Def^KK111656 is a suppressor of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

NOT Suppressor of

Statement

Reference

Scer\GAL4^apolpp.PB/vkg^NIG.16858R is a non-suppressor of fat body phenotype of Scer\GAL4^apolpp.PB, Tango1^NIG.11098R

(Ríos-Barrera et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^apolpp.PB/NimB5^UAS.cRa rescues NimB5^SK1

(Ramond et al., 2020)

Scer\GAL4^apolpp.PB/Mtp^UAS.cPa rescues Mtp^2L3637

(Baer et al., 2012)

Scer\GAL4^apolpp.PB/Mtp^UAS.cPa rescues Mtp^Δex1

(Palm et al., 2012)

Comments

Images (0)

Mutant

Wild-type

Stocks (6)

Bloomington

84317

w^*; P{UAS-GC3Ai}2; P{Lpp-GAL4.B}3

84297

w^*; P{UAS-CC3Ai}2; P{Lpp-GAL4.B}3/TM6B, Tb¹

84338

w^*; P{UAS-VC3Ai}2; P{Lpp-GAL4.B}3/TM6B, Tb¹

67078

w^*; P{UAS-Cas9.P2}attP40/CyO; P{Lpp-GAL4.B}3/TM6B, Tb¹

606651

w^*; P{Lpp-GAL4.B}3, P{UAS-sfGFP-TurboID-ER}attP2/TM3, Sb¹

67061

w^*; P{UAS-3xFLAG.dCas9.VPR}attP40, P{tubP-GAL80^ts}10; P{Lpp-GAL4.B}3/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\GAL4^Rfabg.PB

Scer\GAL4^apolpp.PB

Name Synonyms

Retinoid- and fatty acid-binding glycoprotein promoter construct of Brankatschk

Secondary FlyBase IDs

References (69)

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