FlyBase Allele Report: Dmel\Def[SK3]

General Information

Symbol

Dmel\Def^SK3

Species

D. melanogaster

Name

FlyBase ID

FBal0346807

Feature type

allele

Associated gene

Dmel\Def

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

Mutagen

CRISPR/Cas9

(Hanson et al., 2019)

Progenitor genotype

Cytology

Description

Mutations within Def.

(Hanson et al., 2019)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2R:10,054,494..10,054,502 [-]

Comment:

Nine bases (AGTTTCCGA) are replaced with five bases (TGAAT) causing a frameshift.

Inserted_sequence:

TGAAT

(Hanson et al., 2019)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by dlg1^A40.2

(Parvy et al., 2019)

modeled by scrib¹

(Parvy et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage

(Parvy et al., 2019)

short lived | conditional

(Parvy et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Def^SK3 adults exhibit sensitivity to bacterial infection with Listeria innocua but not Erwinia carotovora.

(Parvy et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Def^SK3 is an enhancer of neoplasia | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Def^SK3 is an enhancer of neoplasia | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Def^SK3 is an enhancer of neoplasia | larval stage phenotype of scrib¹

(Parvy et al., 2019)

Suppressor of

Statement

Reference

Def^SK3 is a suppressor of increased cell death | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Def^SK3 is a suppressor of increased cell death | larval stage phenotype of dlg1^A40.2

(Parvy et al., 2019)

Def^SK3 is a suppressor of increased cell death | larval stage phenotype of scrib¹

(Parvy et al., 2019)

Other

Statement

Reference

AttC^MB05438, AttD^SK1, Def^SK3, Df(2R)Dpt-SK1, Df(2R)Dro-AttAB-SK2, Df(3R)ΔCecA-C, Drs^R1, Mtk^R1 has abnormal immune response | adult stage phenotype

(Hanson et al., 2023)

AttC^MB05438, AttD^SK1, Def^SK3, Df(2R)Dpt-SK1, Df(2R)Dro-AttAB-SK2, Df(3R)ΔCecA-C, Drs^R1, Mtk^R1 has visible | adult stage phenotype

(Hanson et al., 2023)

AttC^MB05438, AttD^SK1, Def^SK3, Df(2R)Dpt-SK1, Df(2R)Dro-AttAB-SK2, Df(3R)ΔCecA-C, Drs^R1, Mtk^R1 has increased mortality | conditional phenotype

(Hanson et al., 2023)

AttC^MB05438, AttD^SK1, Def^SK3, Df(2R)Dpt-SK1, Df(2R)Dro-AttAB-SK2, Drs^R1, Mtk^R1 has abnormal immune response | adult stage phenotype

(Hanson et al., 2023)

AttC^MB05438, AttD^SK1, Def^SK3, Df(2R)Dpt-SK1, Df(2R)Dro-AttAB-SK2, Drs^R1, Mtk^R1 has visible | adult stage phenotype

(Hanson et al., 2023)

AttC^MB05438, AttD^SK1, Def^SK3, Df(2R)Dpt-SK1, Df(2R)Dro-AttAB-SK2, Drs^R1, Mtk^R1 has increased mortality | conditional phenotype

(Hanson et al., 2023)

Phenotype Manifest In

Enhancer of

Statement

Reference

Def^SK3 is an enhancer of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Def^SK3 is an enhancer of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Def^SK3 is an enhancer of wing disc phenotype of scrib¹

(Parvy et al., 2019)

Suppressor of

Statement

Reference

Def^SK3 is a suppressor of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Scer\GAL4^apolpp.PB, Def^UAS.cTa, Def^SK3 is a suppressor of wing disc phenotype of dlg1^A40.2

(Parvy et al., 2019)

Def^SK3 is a suppressor of wing disc phenotype of scrib¹

(Parvy et al., 2019)

Other

Statement

Reference

AttC^MB05438, AttD^SK1, Def^SK3, Df(2R)Dpt-SK1, Df(2R)Dro-AttAB-SK2, Df(3R)ΔCecA-C, Drs^R1, Mtk^R1 has adult abdomen | conditional phenotype

(Hanson et al., 2023)

AttC^MB05438, AttD^SK1, Def^SK3, Df(2R)Dpt-SK1, Df(2R)Dro-AttAB-SK2, Drs^R1, Mtk^R1 has adult abdomen | conditional phenotype

(Hanson et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Def^SK3 enhances the tumor phenotype while suppressing the associated apoptosis (Dcp1 staining) of dlg1^A40.2 wing discs, but does not affect the cell division rate; the additional expression of Def^UAS.cTa under the control of Scer\GAL4^apolpp.PB (in the Def^SK3, dlg1^A40.2 background) partially re-establishes the original phenotypes of dlg1^A40.2.

Injecting synthetic Defensin protein into Def^SK3, dlg1^A40.2 double mutant larvae partially re-establishes the increased apoptosis observed in dlg1^A40.2 larvae.

Def^SK3 enhances the tumor phenotype while suppressing the associated apoptosis (Dcp1 staining) of scrib¹ wing discs

(Parvy et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Def^SK3

(Zhao et al., 2025, Rommelaere et al., 2024, Hanson et al., 2023, Swanson et al., 2020, Hanson et al., 2019)

def^sk3

(Parvy et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close