FlyBase Allele Report: Dmel\Tango1[NIG.11098R]

General Information

Symbol

Dmel\Tango1^NIG.11098R

Species

D. melanogaster

Name

FlyBase ID

FBal0270732

Feature type

allele

Associated gene

Dmel\Tango1

Associated Insertion(s)

Carried in Construct

P{NIG.11098R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21)

Progenitor genotype

Carried in construct

P{NIG.11098R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-11098R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

endoplasmic reticulum exit site, with Scer\GAL4^bs.Term

(Ríos-Barrera et al., 2017)

endoplasmic reticulum exit site | larval stage, with Scer\GAL4^apolpp.PB

(Ríos-Barrera et al., 2017)

endoplasmic reticulum exit site | larval stage, with Scer\GAL4^nub.PK

(Ríos-Barrera et al., 2017)

endoplasmic reticulum exit site | larval stage, with Scer\GAL4^ptc.PU

(Ríos-Barrera et al., 2017)

fat body, with Scer\GAL4^apolpp.PB

(Ríos-Barrera et al., 2017)

Golgi apparatus, with Scer\GAL4^bs.Term

(Ríos-Barrera et al., 2017)

larval brain neuropil glial cell, with Scer\GAL4^repo.PU

(Ríos-Barrera et al., 2017)

secondary tracheal branch, with Scer\GAL4^bs.Term

(Ríos-Barrera et al., 2017)

tendon cell | pupal stage, with Scer\GAL4^sr-md710

(Ríos-Barrera et al., 2017)

terminal tracheal cell, with Scer\GAL4^bs.Term

(Ríos-Barrera et al., 2017)

tracheal section, with Scer\GAL4^bs.Term

(Ríos-Barrera et al., 2017)

wing pouch, with Scer\GAL4^nub.PK

(Ríos-Barrera et al., 2017)

Detailed Description

Statement

Reference

Expression of Tango1^NIG.11098R under the control of Scer\GAL4^bs.Term causes air-filling defects and reduced branch numbers in the trachea terminal cells region. Terminal cells show abnormal ER and Golgi, as compared to controls: abnormal/enlarged ER exit sites (identified by Sec16 staining), which seem to be enclosed by the medial Golgi (identified by ManII-GFP) instead of being juxtaposed; the cis-Golgi (identified by GM130) and trans-Golgi (identified by GalT) shows an abnormal enlarged ring-like structure.

Expression under the control of Scer\GAL4^bs.Term, Scer\GAL4^nub.PK, Scer\GAL4^repo.PU or Scer\GAL4^sr-md710 impairs the secretion of Dpy by tracheal terminal cells, wing disc pouch cells, larval brain glial cells or pupal tendon cells, respectively; expression under the control of Scer\GAL4^repo.PU also leads to the abnormal accumulation of LanA and LanB1 in the cytoplasm of glial cells; expressing under the control of Scer\GAL4^nub.PK or Scer\GAL4^ptc.PU induces a decrease in endoplasmic reticulum exit sites (identified by Sec16) in the wing disc pouch. Expression under the control of Scer\GAL4^apolpp.PB, but not Scer\GAL4^bs.Term, lead to a decrease in the levels of Collagen-IV surrounding tracheal terminal cells; expression under the control of Scer\GAL4^apolpp.PB leads to accumulation of Collagen-IV, LanA, LanB1 and SPARC, as well as to endoplasmic reticulum exit site aggregates (identified by Sec16) in the larval fat body; both of these results indicate a defect in secretion by the fat body.