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FB2026_01
,
released March 12, 2026
Imprecise excision of P{GSV2}GS50262 has resulted in a 2,509bp deletion that removes the 5'UTR of Patj as well as the L27 motif and the first three PDZ domains.
2509 bp deletion resulting from the imprecise excision of P{GSV2}GS50262.
No discernible defects are seen in the cellularising cells of stage 5 embryos derived from females carrying PatjΔ7 germline clones. The embryos complete their development through the embryonic and larval stages. The overall pattern of apical-basal polarity in stage 10 and 11 embryos appears normal.
No apical-basal polarity defects are seen in PatjΔ7 mutant larval imaginal discs or adult follicular cell clones.
PatjΔ7 mutant photoreceptor clones in the early pupal retina show apical-basal polarity defects and the adherens junctions appear to be disrupted.
No obvious morphological abnormalities are observed in PatjΔ7/Df(3L)BSC123 mutant larvae. However, within several hours of pupal formation the PatjΔ7/Df(3L)BSC123 pupae exhibit deformities in shape, with a flattened anterior end and rounded posterior. At 16% pupal development the head region is underdeveloped, although the imaginal discs appear to have successfully everted. Older PatjΔ7/Df(3L)BSC123 pupae fail to develop head and thoracic regions and growth of the everted imaginal discs is stalled.
Expression of Patj+tL rescues the lethality and pupal defects seen in PatjΔ7 mutants.
Expression of Patj+tL rescues the lethality seen in PatjΔ7/PatjΔ13 mutants.
Expression of Patj+tL rescues the lethality and pupal defects seen in PatjΔ7/Df(3L)BSC123 mutants.
Expression of PatjS.T:Avic\GFP rescues the lethality and pupal defects seen in PatjΔ7 mutants.
Expression of PatjS.T:Avic\GFP rescues the lethality seen in PatjΔ7/PatjΔ13 mutants.
Expression of PatjS.T:Avic\GFP rescues the lethality seen in PatjΔ7/Df(3L)BSC123 mutants.
Expression of PatjS.T:Avic\GFP rescues the lethality seen in flies that are both maternally and zygotically mutant for PatjΔ7 (generated using the ovoD system).