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General Information
Symbol
Dmel\Nlg1UAS.cMa
Species
D. melanogaster
Name
FlyBase ID
FBal0279830
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dnlg1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Nlg1 coding sequences that correspond to cDNA clone RE29404.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Nlg1Scer\UAS.cMa under the control of Scer\GAL4Mef2.PU does not lead to significant changes in the number of synaptic boutons at the third instar larval neuromuscular junction, as compared to controls.

Driving expression of wild-type Nlg1Scer\UAS.cMa in the muscle using Scer\GAL4BG487 increases bouton number above wild-type levels by greater than 40%.

Overexpression of Nlg1Scer\UAS.cMa in the muscles of wild-type larvae (under the control of Scer\GAL4BG487) reduces mEJC amplitude by 25% and EJC amplitude by over 60%.

Nlg1Scer\UAS.cMa overexpression in muscle (under the control of Scer\GAL4BG487) causes synaptic overgrowth and inhibition of glutamate release.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Synaptic overgrowth caused by Nlg1Scer\UAS.cMa overexpression in the muscle (under the control of Scer\GAL4BG487) is suppressed by Nrx-1273.

Overexpression of Nlg1Scer\UAS.cMa in a Nrx-1273 mutant background does not increase bouton number above that of the negative control (Scer\GAL4BG487/Nlg1Scer\UAS.cMa; Nrx-1273/+).

The inhibitory effects of Nlg1Scer\UAS.cMa overexpression on evoked synaptic transmission are suppressed in Nrx-1273 mutants. EJC amplitude is also significantly restored in these flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

The expression of Nlg1Scer\UAS.cMa under the control of Scer\GAL4Mef2.PU rescues the decreased number of synaptic boutons observed at the Nlg1Δ46 homozygous third instar larval neuromuscular junction.

Driving expression of wild-type Nlg1Scer\UAS.cMa in the muscles of Nlg1Δ46 mutants using Scer\GAL4BG487 increases bouton number above wild-type levels by greater than 40%.

Unlike bouton number, EJC amplitude in Nlg1Δ46 mutants is not restored by postsynaptic expression of Nlg1Scer\UAS.cMa.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Nlg1Scer\UAS.cMa
Nlg1UAS.cMa
Name Synonyms
Secondary FlyBase IDs
    References (2)