FlyBase Gene Report: Dmel\Nlg1

General Information

Symbol

Dmel\Nlg1

Species

D. melanogaster

Name

Neuroligin 1

Annotation Symbol

CG31146

Feature Type

protein_coding_gene

FlyBase ID

FBgn0051146

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Neuroligin 1 (Nlg1) encodes a transsynaptic adhesion protein of the neuroligin family that is expressed in muscles and accumulates at the postsynaptic side of neuromuscular junctions. Lack of Nlg1 causes smaller and underdeveloped neuromuscular junctions due to insufficient postsynaptic maturation and differentiation of synaptic boutons. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

dnlg1, neuroligin

Key Links

Genomic Location

Cytogenetic map

84D11-84D12

Sequence location

3R:7,658,247..7,694,874 [+]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:7658247..7694874

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables cell-cell adhesion mediator activity

inferred from direct assay

(Banerjee et al., 2017)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables neurexin family protein binding

inferred from biological aspect of ancestor with PANTHER:PTN000168538

(GO Reference Genome Project, 2011-)

enables signaling receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000168538

(GO Reference Genome Project, 2011-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in neurexin clustering involved in presynaptic membrane assembly

inferred from mutant phenotype

(Banerjee et al., 2017)

involved_in neuromuscular junction development

inferred from mutant phenotype

(Banovic et al., 2010)

involved_in synaptic assembly at neuromuscular junction

inferred from mutant phenotype

(Banerjee et al., 2017, Owald et al., 2012)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

involved_in chemical synaptic transmission

inferred from biological aspect of ancestor with PANTHER:PTN000168538

(GO Reference Genome Project, 2011-)

involved_in modulation of chemical synaptic transmission

inferred from biological aspect of ancestor with PANTHER:PTN000168538

(GO Reference Genome Project, 2011-)

involved_in neuron cell-cell adhesion

inferred from biological aspect of ancestor with PANTHER:PTN000168538

(GO Reference Genome Project, 2011-)

involved_in postsynaptic membrane assembly

inferred from biological aspect of ancestor with PANTHER:PTN000168538

(GO Reference Genome Project, 2011-)

involved_in presynaptic membrane assembly

inferred from biological aspect of ancestor with PANTHER:PTN000168538

(GO Reference Genome Project, 2011-)

involved_in synaptic vesicle endocytosis

inferred from biological aspect of ancestor with PANTHER:PTN000168538

(GO Reference Genome Project, 2011-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in neuromuscular junction

inferred from direct assay

(Banovic et al., 2010)

located_in postsynaptic membrane

inferred from direct assay

(Banerjee et al., 2017)

part_of trans-synaptic protein complex

inferred from physical interaction with FLYBASE:Nrx-1; FB:FBgn0038975

(Banerjee et al., 2017)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in cell surface

inferred from biological aspect of ancestor with PANTHER:PTN000168538

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000168538

(GO Reference Genome Project, 2011-)

is_active_in postsynaptic membrane

inferred from biological aspect of ancestor with PANTHER:PTN002889381

(GO Reference Genome Project, 2011-)

is_active_in synapse

inferred from biological aspect of ancestor with PANTHER:PTN000168538

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Summary (Interactive Fly)

Postsynaptic receptor that interacts with presynaptic Neurexins - recruits scaffolding proteins for organization of postsynaptic neurotransmitter receptors - mediates the precise apposition of presynaptic and postsynaptic membranes

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Nlg1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VIC7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.54

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nlg1-RD

FBtr0081541

NM_169191

6006

1354

Nlg1-RE

FBtr0305969

NM_001260037

5691

1354

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Nlg1-PD

FBpp0081065

149.7

1354

6.54

FBpp0297111

149.7

1354

6.54

Q9VIC7

NP_001246966

AFH06285

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1354 aa isoforms: Nlg1-PD, Nlg1-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VIC7

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nlg1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Nlg1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.84

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

dorsal pharyngeal muscle cell | subset

(Banovic et al., 2010)

ring gland

(Banovic et al., 2010)

embryonic stage 12 -- 14

embryonic myoblast | subset

(Banovic et al., 2010)

embryonic stage 14

larval somatic muscle cell | subset

(Banovic et al., 2010)

Additional Descriptive Data

Expression of Nlg1 is first detected at late stage 12 in a subset of myoblasts, and is detected in most myoblasts by stage 14. By the end of embryogenesis, Nlg1 is expressed in the dorsal pharyngeal muscles and the ring gland.

(Banovic et al., 2010)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval neuromuscular junction

• postsynaptic membrane | punctate

(Mozer and Sandstrom, 2012)

embryonic/larval neuromuscular junction

Comment: postsynaptically localized

(Banovic et al., 2010)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval hypodermal muscle cell

(Mozer and Sandstrom, 2012)

Additional Descriptive Data

Nlg1 is expressed in the postsynaptic part of the neuromuscular junction, located adjacent to the postsynaptic density.

(Banovic et al., 2010)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in neuromuscular junction

inferred from direct assay

(Banovic et al., 2010)

located_in postsynaptic membrane

inferred from direct assay

(Banerjee et al., 2017)

part_of trans-synaptic protein complex

inferred from physical interaction with FLYBASE:Nrx-1; FB:FBgn0038975

(Banerjee et al., 2017)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Nlg1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Nlg1

View exonic expression by tissue for Dmel\Nlg1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0051146

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40913/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051146

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051146

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051146

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31146

Flygut - An atlas of the Drosophila adult midgut

FBgn0051146

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 25 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Nlg1

Transgenic constructs containing regulatory region of Nlg1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Nlg1^ex3.1

(Banovic et al., 2010)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Scer\GAL4^Mef2.PR

Nlg1^GD2848

viable, with Scer\GAL4^pnr-MD237

Nlg1^GD2848

viable, with Scer\GAL4^tin.CΔ4

Nlg1^GD2848

viable (with Df(3R)BSC747)

viable (with Nlg1^ex2.3)

Nlg1^I960

viable (with Nlg1^I960)

Nlg1^ex2.3

Sterility

Allele

fertile

Nlg1^PL00132

Other Phenotypes

Allele

abnormal locomotor behavior | adult stage (with Df(3R)BSC747)

Nlg1^ex2.3

abnormal neuroanatomy

abnormal neuroanatomy, with Scer\GAL4^BG487

Nlg1^UAS.cMa

abnormal neuroanatomy, with Scer\GAL4^Mef2.PR

Nlg1^{Δcyto.UAS.EGFP}

abnormal neuroanatomy (with Df(3R)ED5223)

Nlg1^Δ46

abnormal neuroanatomy | larval stage

Nlg1^I960

abnormal neuroanatomy | larval stage, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

abnormal neuroanatomy | larval stage (with Df(3R)dsx29)

Nlg1^I960

abnormal neuroanatomy | larval stage (with Nlg1^ex1.9)

Nlg1^ex2.3

abnormal neuroanatomy | larval stage (with Nlg1^ex2.3)

abnormal neuroanatomy | larval stage (with Nlg1^H324)

Nlg1^I960

abnormal neuroanatomy | larval stage (with Nlg1^I960)

abnormal neuroanatomy | pharate adult stage, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

abnormal neuroanatomy | pharate adult stage, with Scer\GAL4^ppk.PG

Nlg1^UAS.cBa

abnormal neuroanatomy | pharate adult stage (with Df(3R)BSC747)

abnormal neuroanatomy | pharate adult stage | somatic clone (with Df(3R)BSC747)

Nlg1^{MI02059-FlpStop.Dlock}

abnormal neuroanatomy | pupal stage P7, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

abnormal neuroanatomy | pupal stage P7 (with Df(3R)BSC747)

abnormal neuroanatomy | third instar larval stage

Nlg1^unspecified

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Mef2.PR

Nlg1^{Δcyto.UAS.EGFP}

abnormal neuroanatomy | third instar larval stage (with Nlg1^ex1.9)

Nlg1^ex2.3

abnormal neuroanatomy | third instar larval stage (with Nlg1^ex2.3)

Nlg1^ex1.9

abnormal neurophysiology

Nlg1^Δ46

abnormal neurophysiology, with Scer\GAL4^BG487

Nlg1^UAS.cMa

abnormal neurophysiology (with Df(3R)ED5223)

Nlg1^Δ46

abnormal neurophysiology | larval stage (with Nlg1^H324)

Nlg1^I960

abnormal neurophysiology | larval stage (with Nlg1^I960)

Nlg1^H324

abnormal neurophysiology | pharate adult stage, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

abnormal size | pharate adult stage, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

abnormal size | pharate adult stage (with Df(3R)BSC747)

abnormal size | pharate adult stage | somatic clone (with Df(3R)BSC747)

Nlg1^{MI02059-FlpStop.Dlock}

abnormal size | pupal stage P7, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

decreased size | third instar larval stage (with Nlg1^ex1.9)

Nlg1^ex2.3

decreased size | third instar larval stage (with Nlg1^ex2.3)

Nlg1^ex1.9

Phenotype manifest in

Allele

abdominal anterior ventral multidendritic neuron vdaa | pharate adult stage, with Scer\GAL4^ppk.PG

Nlg1^UAS.cBa

abdominal lateral tergosternal muscle cell | pharate adult stage, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

abdominal lateral tergosternal muscle cell | pharate adult stage (with Df(3R)BSC747)

abdominal lateral tergosternal muscle cell | pharate adult stage | somatic clone, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

abdominal lateral tergosternal muscle cell | pharate adult stage | somatic clone (with Df(3R)BSC747)

Nlg1^{MI02059-FlpStop.Dlock}

abdominal lateral tergosternal muscle cell | pupal stage P7, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

abdominal lateral tergosternal muscle cell | pupal stage P7 (with Df(3R)BSC747)

abdominal lateral tergosternal muscle cell | pupal stage P7 | somatic clone, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

adult motor neuron (with Df(3R)BSC747)

Nlg1^ex2.3

adult persistent motor neuron | pharate adult stage

Nlg1^I960

adult persistent motor neuron | pharate adult stage, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

adult persistent motor neuron | pharate adult stage, with Scer\GAL4^ppk.PG

Nlg1^UAS.cBa

adult persistent motor neuron | pharate adult stage (with Df(3R)BSC747)

adult persistent motor neuron | pharate adult stage | somatic clone, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

adult persistent motor neuron | pharate adult stage | somatic clone (with Df(3R)BSC747)

Nlg1^{MI02059-FlpStop.Dlock}

adult persistent motor neuron | pupal stage P7, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

adult persistent motor neuron | pupal stage P7 (with Df(3R)BSC747)

adult persistent motor neuron | pupal stage P7 | somatic clone, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

bouton

Nlg1^Δ46

bouton, with Scer\GAL4^BG487

Nlg1^UAS.cMa

bouton (with Df(3R)ED5223)

Nlg1^Δ46

cytoskeleton of presynaptic active zone | third instar larval stage

Nlg1^unspecified

distal axon | pharate adult stage, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

distal axon | pharate adult stage (with Df(3R)BSC747)

distal axon | pharate adult stage | somatic clone, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

distal axon | pharate adult stage | somatic clone (with Df(3R)BSC747)

Nlg1^{MI02059-FlpStop.Dlock}

distal axon | pupal stage P7, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

distal axon | pupal stage P7 (with Df(3R)BSC747)

distal axon | pupal stage P7 | somatic clone, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

embryonic/larval neuromuscular junction | larval stage (with Nlg1^ex1.9)

Nlg1^ex2.3

embryonic/larval neuromuscular junction | larval stage (with Nlg1^ex2.3)

Nlg1^ex1.9

embryonic/larval neuromuscular junction | third instar larval stage (with Nlg1^ex1.9)

Nlg1^ex2.3

embryonic/larval neuromuscular junction | third instar larval stage (with Nlg1^ex2.3)

Nlg1^ex1.9

filopodium | pharate adult stage, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

filopodium | pupal stage P7, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

growth cone | pupal stage P7, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

muscle cell of A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Nlg1^ex1.9)

Nlg1^ex2.3

muscle cell of A1-7 lateral longitudinal muscle 1 | third instar larval stage (with Nlg1^ex2.3)

Nlg1^ex1.9

neuromuscular junction

neuromuscular junction, with Scer\GAL4^Mef2.PR

Nlg1^{Δcyto.UAS.EGFP}

neuromuscular junction | larval stage

Nlg1^I960

neuromuscular junction | larval stage, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

neuromuscular junction | larval stage (with Nlg1^ex2.3)

Nlg1^I960

neuromuscular junction | larval stage (with Nlg1^H324)

Nlg1^I960

neuromuscular junction | larval stage (with Nlg1^I960)

neuromuscular junction | third instar larval stage

Nlg1^Δ46

neuromuscular junction | third instar larval stage, with Scer\GAL4^Mef2.PU

Nlg1^{Δcyto.UAS.EGFP}

NMJ bouton (with Nlg1^ex2.3)

Nlg1^I960

NMJ bouton (with Nlg1^I960)

Nlg1^ex2.3

NMJ bouton | larval stage

Nlg1^I960

NMJ bouton | larval stage (with Df(3R)dsx29)

Nlg1^I960

NMJ bouton | larval stage (with Nlg1^ex2.3)

Nlg1^I960

NMJ bouton | larval stage (with Nlg1^H324)

Nlg1^I960

NMJ bouton | larval stage (with Nlg1^I960)

NMJ bouton | third instar larval stage

Nlg1^unspecified

NMJ bouton | third instar larval stage, with Scer\GAL4^Mef2.PR

Nlg1^{Δcyto.UAS.EGFP}

ovariole, with Scer\GAL4^tj.PU

Nlg1^KK104381

postsynaptic density | third instar larval stage

Nlg1^Δ46

presynaptic active zone (with Nlg1^ex1.9)

Nlg1^ex2.3

presynaptic active zone (with Nlg1^ex2.3)

Nlg1^ex1.9

presynaptic active zone | adult stage, with Scer\GAL4^Mef2.PR

Nlg1^UAS.cBa

presynaptic active zone | decreased number (with Nlg1^ex1.9)

Nlg1^ex2.3

presynaptic active zone | decreased number (with Nlg1^ex2.3)

Nlg1^ex1.9

presynaptic active zone | ectopic | pharate adult stage, with Scer\GAL4^ppk.PG

Nlg1^UAS.cBa

presynaptic active zone | third instar larval stage

Nlg1^Δ46

subsynaptic reticulum (with Nlg1^H324)

Nlg1^I960

subsynaptic reticulum (with Nlg1^I960)

Nlg1^H324

subsynaptic reticulum | third instar larval stage

Nlg1^Δ46

synapse

Nlg1^Δ46

synapse, with Scer\GAL4^BG487

Nlg1^UAS.cMa

synapse (with Df(3R)ED5223)

Nlg1^Δ46

terminal bouton | third instar larval stage

Nlg1^Δ46

terminal bouton | third instar larval stage, with Scer\GAL4^Mef2.PU

Nlg1^{Δcyto.UAS.EGFP}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (15)

Hsap\NLGN1

6 of 14

Yes

Hsap\NLGN2

6 of 14

Yes

Hsap\NLGN3

6 of 14

Yes

Hsap\NLGN4X

6 of 14

Yes

5 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\TG

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (26)

Rnor\Nlgn3

6 of 14

Yes

5 of 14

5 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (27)

Mmus\Nlgn2

6 of 14

Yes

Mmus\Nlgn3

6 of 14

Yes

5 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (14)

Xtro\nlgn3

4 of 13

Yes

Xtro\nlgn4x

4 of 13

Yes

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\tg

1 of 13

Danio rerio (Zebrafish) (18)

Drer\nlgn1

6 of 14

Yes

Drer\nlgn2a

6 of 14

Yes

Drer\nlgn2b

6 of 14

Yes

Drer\nlgn3b

6 of 14

Yes

Drer\nlgn4xa

6 of 14

Yes

5 of 14

5 of 14

4 of 14

4 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Drer\si:dkey-30c15.17

2 of 14

Drer\LOC100334697

1 of 14

Drer\si:ch211-71n6.4

1 of 14

Drer\si:dkey-193c22.1

1 of 14

Drer\tg

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (45)

Cele\nlg-1

6 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\ZK994.1

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (48)

Agam\AgaP_AGAP003570

11 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (3)

Atha\ICME-LIKE1

1 of 13

Yes

Atha\ICME-LIKE2

1 of 13

Yes

Atha\PCME

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\SPCC417.12

4 of 12

Yes

Spom\SPAC8F11.08c

1 of 12

Yes

Spom\SPAPB18E9.04c

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Nlg1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (34)

Gli

6 of 13

6 of 13

6 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

gas

5 of 13

Jhedup

5 of 13

Jhe

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

clt

4 of 13

Est-P

4 of 13

Ace

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

Nrt

2 of 13

CG30095

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

Nlg1^ex1.9

model of autism spectrum disorder

CEA

(Guangming et al., 2023)

CEA with Nlg1^ex2.3

(Sun et al., 2023)

Nlg1^ex2.3

model of autism spectrum disorder

CEA

(Guangming et al., 2023)

CEA with Nlg1^ex1.9

(Sun et al., 2023)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NLGN1; neuroligin 1

6 of 14

NEUROLIGIN 1; NLGN1

AUTISM, SUSCEPTIBILITY TO, 20; AUTS20

6 of 14

6 of 14

AUTISM, SUSCEPTIBILITY TO, X-LINKED 1; AUTSX1

NLGN4X; neuroligin 4 X-linked

6 of 14

300427

AUTISM, SUSCEPTIBILITY TO, X-LINKED 2; AUTSX2

NLGN4Y; neuroligin 4 Y-linked

5 of 14

400028

CEL; carboxyl ester lipase

4 of 14

114840

MATURITY-ONSET DIABETES OF THE YOUNG, TYPE 8, WITH EXOCRINE DYSFUNCTION; MODY8

maturity-onset diabetes of the young type 8

CES1; carboxylesterase 1

3 of 14

114835

DRUG METABOLISM, ALTERED, CES1-RELATED

CES2; carboxylesterase 2

3 of 14

605278

CES3; carboxylesterase 3

3 of 14

605279

CES4A; carboxylesterase 4A

3 of 14

621153

CES5A; carboxylesterase 5A

3 of 14

618678

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Nlg1 - Nrx-1

anti bait coimmunoprecipitation, western blot

(Banerjee et al., 2017)

Nlg1 - SCAR

pull down, western blot

(Xing et al., 2018)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Nlg1

suppressible

Nrx-1

(Mozer and Sandstrom, 2012, Owald et al., 2012)

Nlg1

enhanceable

wit

(Banerjee et al., 2017)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Nlg2

suppressible

Nlg1

(Ramesh et al., 2021)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0051146

MIST (genetic) - An integrated Molecular Interaction Database

40913

MIST (protein-protein) - An integrated Molecular Interaction Database

40913

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84D11-84D12

Sequence location

3R:7,658,247..7,694,874 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84D11-84D12

Limits computationally determined from genome sequence between P{EP}EP3060^EP3060 and P{PZ}grn⁰⁵⁹³⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

78859

w^*; Nlg1^Δ46/TM6B, Tb¹

40883

y¹ v¹; P{TRiP.HMS02131}attP40/CyO

23176

y¹ w^67c23; Mi{ET1}Nlg1^MB00987

607154

w^*; P{Mhc.CD8-GFP-Sh}3 Nlg1^H703/TM6B, Tb¹

81419

y¹ sc^* v¹ sev²¹; P{SAM.dCas9.GS04053}attP40

78860

w^*; PBac{Nlg1^+tMa}VK00037; Nlg1^Δ46/TM6B, Tb¹

32820

y¹ w^*; Mi{MIC}Nlg1^MI02059/TM3, Sb¹ Ser¹

44137

y¹ w^*; Mi{MIC}Nlg1^MI03763/TM3, Sb¹ Ser¹

19622

w^*; P{FRT(w^hs)}2A P{neoFRT}82B PBac{GAL4D,EYFP}Nlg1^PL00132

Korea

13164

P{VT038018-GAL4}attP2

VDRC

v104209

P{KK104381}VIE-260B

v42616

w¹¹¹⁸; P{GD2848}v42616

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (7)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0051146

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RE29628

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0051146

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Mozer and Sandstrom, 2012, Banovic et al., 2010)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Area matching Drosophila EST AA141365 (inverted).

(Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG31146 anon-WO0118547.256

(FlyBase, 1996-)

Source for merge of: CG12148 CG14596

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG12148 and CG14596 merged as CG31146 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of CG31146 anon-WO0118547.256 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Nlg1 CG31146

(Banovic et al., 2010)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG12148

CG14596

CG31146

(Ashwal-Fluss et al., 2014, Li et al., 2013, Mozer and Sandstrom, 2012, Sun et al., 2011, Banovic et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Haerty et al., 2007, Lipatov et al., 2005)

DNlg1

(Xing et al., 2018, Li et al., 2013)

Nlg

(Turrel et al., 2024)

Nlg-1

(Harris and Littleton, 2015)

anon-WO0118547.256

dnlg1

(Sun et al., 2023, Xing et al., 2018, Banerjee et al., 2017, Mozer and Sandstrom, 2012, Owald et al., 2012, Banovic et al., 2010)

neuroligin

(Kamimura and Maeda, 2021)

nlg1

(Gunderson et al., 2021, Ramesh et al., 2021, Chou et al., 2020, Guangming et al., 2020, Sulkowski et al., 2016, Mosca et al., 2012, Mosca et al., 2012)

Name Synonyms

Neuroligin 1

(Mosca et al., 2012)

Neuroligin-1

(Yamagata, 2020)

neuroligin

(Baxley et al., 2017, Soshnev et al., 2013, Owald et al., 2012)

neuroligin 1

(Gunderson et al., 2021, Mozer and Sandstrom, 2012, Sun et al., 2011, Banovic et al., 2010)

Secondary FlyBase IDs

FBgn0037510
FBgn0037511
FBgn0062140

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40913

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VIC7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VIC7

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VIC7

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0051146

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40913/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0051146

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0051146

FlyMine - An integrated database for Drosophila genomics

FBgn0051146

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG31146

MARRVEL_MODEL - MARRVEL (model organism gene)

40913

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0051146

DroID - A comprehensive database of gene and protein interactions.

FBgn0051146

DRSC - Results frm RNAi screens

FBgn0051146

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Nlg1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0051146

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0051146

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31146

Flygut - An atlas of the Drosophila adult midgut

FBgn0051146

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0051146

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC033027

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

40913

MIST (protein-protein) - An integrated Molecular Interaction Database

40913

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (82)

The following external sites may use different assemblies or annotations than FlyBase.

-0.84

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