FlyBase Allele Report: Dmel\Fak[RNAi.UAS]

General Information

Symbol

Dmel\Fak^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0280004

Feature type

allele

Associated gene

Dmel\Fak

Associated Insertion(s)

Carried in Construct

P{UAS-Fak.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Tsai et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Tsai et al., 2008)

Mutagen

in vitro construct

(Tsai et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Fak.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a cDNA fragment corresponding to nucleotides 629-1177 of Fak.

(Tsai et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Fak

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav.PU

(Tsai et al., 2012)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PU

(Tsai et al., 2008)

Phenotype Manifest In

neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PU

(Tsai et al., 2008)

NMJ bouton | increased number | third instar larval stage, with Scer\GAL4^elav.PU

(Tsai et al., 2008)

NMJ bouton | larval stage | increased number, with Scer\GAL4^elav.PU

(Tsai et al., 2012)

Detailed Description

Statement

Reference

Expression of Fak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav.PU results in an increase in bouton number per muscle area at the neuromuscular junction.

(Tsai et al., 2012)

Significant overgrowth of the neuromuscular junction (NMJ) (increased bouton number per muscle area and increased NMJ length per muscle area) is seen in third instar larvae expressing Fak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav.PU, while expression under the control of Scer\GAL4^Mhc.PW has no effect on NMJ growth.

(Tsai et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Fak^RNAi.UAS, Scer\GAL4^elav.PU has abnormal neuroanatomy | larval stage phenotype, suppressible by Pde4^dnc-M14/dnc[+]

(Tsai et al., 2012)

Fak^RNAi.UAS, Scer\GAL4^elav.PU has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by rl[+]/rl¹⁰

(Tsai et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Fak^RNAi.UAS, Scer\GAL4^elav.PU has NMJ bouton | increased number | larval stage phenotype, suppressible by Pde4^dnc-M14/dnc[+]

(Tsai et al., 2012)

Fak^RNAi.UAS, Scer\GAL4^elav.PU has neuromuscular junction | third instar larval stage phenotype, suppressible by rl[+]/rl¹⁰

(Tsai et al., 2008)

Fak^RNAi.UAS, Scer\GAL4^elav.PU has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by rl[+]/rl¹⁰

(Tsai et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

dnc^M14/+ suppresses the increase in bouton number per muscle area at the neuromuscular junction seen in larvae expressing Fak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav.PU.

(Tsai et al., 2012)

rl¹⁰/+ completely suppresses the overgrowth of the neuromuscular junction (increased bouton number per muscle area and increased NMJ length per muscle area) seen in third instar larvae expressing Fak^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav.PU.

(Tsai et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Fak^RNAi.UAS

Fak^{dsRNA.Scer\UAS}

Fak^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close