FlyBase Allele Report: Dmel\loqs[PA.Tag:FLAG]

General Information

Symbol

Dmel\loqs^PA.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0280144

Feature type

allele

Associated gene

Dmel\loqs

Associated Insertion(s)

Carried in Construct

P{loqs-PA.FLAG}

Key Links

Transgenic product class

cDNA

(Fukunaga et al., 2012)

wild_type

(Fukunaga et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Fukunaga et al., 2012)

wild_type

(Fukunaga et al., 2012)

Mutagen

in vitro construct

(Fukunaga et al., 2012)

Progenitor genotype

Carried in construct

P{loqs-PA.FLAG}

Cytology

Description

loqs promoter and regulatory regions (2.5 kb upstream and 0.8 kb downstream of the locus) drive expression of a cDNA encoding the loqs-PA isoform with a 3xTag:FLAG flag added to the amino-terminus of the protein.

(Fukunaga et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

loqs

Encoded product / tool

loqs

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with loqs^KO

(Fukunaga et al., 2012)

female sterile, with loqs^KO, loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

male fertile, with loqs^KO

(Fukunaga et al., 2012)

viable, with loqs^KO

(Fukunaga et al., 2012)

Phenotype Manifest In

dorsal appendage, with loqs^KO

(Fukunaga et al., 2012)

dorsal appendage, with loqs^KO, loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

germline stem cell, with loqs^KO, loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

Detailed Description

Statement

Reference

loqs^{PA.T:Zzzz\FLAG} restores the embryonic viability of loqs^KO mutants. The rescued males are fertile, however, females lay far fewer eggs than wild-type controls. None of the eggs produced by loqs^KO mutants rescued by loqs^{PA.T:Zzzz\FLAG} hatch, and few of the eggs display normal dorsal appendages.

loqs^KO mutant females rescued by loqs^{PA.T:Zzzz\FLAG} together with loqs^{PD.T:Zzzz\FLAG} lay far fewer eggs than wild-type controls. None of the eggs produced by the rescued females hatch. Few of the eggs laid by the rescued females display normal appendages.

loqs^KO mutant females rescued by either loqs^{PA.T:Zzzz\FLAG} or the combination of loqs^{PA.T:Zzzz\FLAG} and loqs^{PD.T:Zzzz\FLAG} display fewer germline stem cells (GSCs) per germaria than wild-type controls.

(Fukunaga et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

loqs^PA.Tag:FLAG/loqs^KO is a suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^PB.Tag:FLAG, loqs^PA.Tag:FLAG, loqs^KO is a suppressor of visible phenotype of w^RNAi.GMR