FlyBase Allele Report: Dmel\Patj[Δ1]

General Information

Symbol

Dmel\Patj^Δ1

Species

D. melanogaster

Name

FlyBase ID

FBal0280202

Feature type

allele

Associated gene

Dmel\Patj

Associated Insertion(s)

TI{TI}Patj^Δ1

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Sen et al., 2012)

Mutagen

I-SceI

(Sen et al., 2012)

gene targeting by homologous recombination

(Sen et al., 2012)

Progenitor genotype

Associated Insertion(s)

TI{TI}Patj^Δ1

Cytology

Description

The Patj open reading frame has been replaced by a mini-w marker.

(Sen et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Sen et al., 2012)

Phenotype Manifest In

embryonic/first instar larval cuticle

(Sen et al., 2012)

Detailed Description

Statement

Reference

Approximately 25% of homozygotes (lacking zygotic Patj function) do not hatch, approximately 25% die in early larval stages and the rest die as early pupae (metamorphosis is not initiated). Homozygous embryos derived from females containing homozygous germline clones (lacking maternal and zygotic Patj function) show nearly the same pattern of lethality as embryos lacking zygotic Patj function, but show an increased lethality in third instar larvae at the expense of dead pupae. Embryos derived from females containing homozygous germline clones mated to wild-type males develop until adulthood.

46% of homozygous embryos that die during embryogenesis have a normal cuticle, more than 30% fail to develop any cuticle and 18% have strong cuticle defects, showing a general shortening of the cuticle and head defects, while segmentation appears normal. Approximately 15% of homozygous embryos that die during embryogenesis fail to retract the germband correctly.

Homozygous follicle cell clones do not show loss of polarity or multilayering of tissue.

(Sen et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Patj^Δ1 has lethal phenotype, enhanceable by shg[+]/shg^R69

(Sen et al., 2012)

Suppressed by

Statement

Reference

Patj^Δ1 has lethal phenotype, suppressible | partially by Mbs^T541/Mbs[+]

(Sen et al., 2012)

Patj^Δ1 has lethal phenotype, suppressible | partially by sqh^UASp.EGFP/Scer\GAL4^arm.PS

(Sen et al., 2012)

NOT suppressed by

Statement

Reference

Patj^Δ1 has lethal phenotype, non-suppressible by Scer\GAL4^arm.PS/sqh^AA.UASp.EGFP

(Sen et al., 2012)

Other

Statement

Reference

Patj^Δ1, shg[+]/shg^R69 has increased cell death | embryonic stage phenotype

(Sen et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Patj^Δ1 has embryonic/first instar larval cuticle phenotype, enhanceable by shg[+]/shg^R69

(Sen et al., 2012)

Suppressed by

Statement

Reference

Patj^Δ1 has embryonic/first instar larval cuticle phenotype, suppressible | partially by Mbs^T541/Mbs[+]

(Sen et al., 2012)

Patj^Δ1 has embryonic/first instar larval cuticle phenotype, suppressible | partially by sqh^UASp.EGFP/Scer\GAL4^arm.PS

(Sen et al., 2012)

Other

Statement

Reference

Patj^Δ1, shg[+]/shg^R69 has embryonic epidermis phenotype

(Sen et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

shg^R69/+ enhances the lethality of Patj^Δ1 homozygotes, with greater lethality at earlier stages. The frequency of cuticle defects seen in Patj^Δ1 embryos is also enhanced by shg^R69/+. Late-stage shg^R69/+ ; Patj^Δ1/Patj^Δ1 embryos have a multilayered epidermis, many of the epidermal cells start to round up and many epidermal cells undergo apoptosis.

Mbs^T541/+ reduces the frequency of embryonic lethality in Patj^Δ1 homozygotes from 28 to 12%. In addition, the cuticle defects seen in Patj^Δ1 embryos are strongly decreased in frequency in the Mbs^T541/+ background. Patj^Δ1/Mbs^T541 Patj^Δ1 pupae start metamorphosis (reflected by elongation and remodelling of the wing disc).

Expression of sqh^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^arm.PS decreases the frequency of embryonic lethality and also decreases embryonic cuticle defects in Patj^Δ1 homozygotes.

Expression of sqh^{AA.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^arm.PS does not the affect the lethality seen in Patj^Δ1 homozygotes.

(Sen et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PATJ^Δ1

(Sen et al., 2015, Sen et al., 2012)

Patj^Δ1

(Wang et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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