FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\mir-12412
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General Information
Symbol
Dmel\mir-12412
Species
D. melanogaster
Name
FlyBase ID
FBal0280289
Feature type
allele
Associated gene
Dmel\mir-124
Associated Insertion(s)
TI{TI}mir-12412
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Sun et al., 2012)
Mutagen
ends-out gene targeting
(Sun et al., 2012)
Progenitor genotype
Associated Insertion(s)
TI{TI}mir-12412
Cytology
Description

The mir-124 hairpin sequence has been deleted. A single loxP site is present in place of the deleted sequence.

(Sun et al., 2012)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mutants have normal external morphology, but are not easily kept as homozygous stocks.

      mir-1246/mir-12412 males have a substantially shortened lifespan compared to controls.

      Homozygous and mir-1246/mir-12412 larvae show defects in locomotion, both in terms of total distance travelled and crawling speed.

      The number of boutons at the neuromuscular junction (NMJ) and NMJ area (normalised to muscle surface area) are normal in homozygous third instar larvae. The amplitude of miniature excitatory junctional currents (mEJCs) is normal, but the evoked EJC shows an increase in amplitude compared to wild type, resulting in an increase in quantal content at the mutant NMJ.

      (Sun et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      mir-1246/mir-12412 is rescued by mir-124+tCH322-39N16

      (Sun et al., 2012)

      mir-12412 is rescued by mir-124+tCH322-39N16

      (Sun et al., 2012)
      Comments

      mir-124+tCH322-39N16 rescues the shortened lifespan of mir-1246/mir-12412 males and the locomotor defects of mir-1246/mir-12412 larvae.

      mir-124+tCH322-39N16 rescues the electrophysiological defects at the neuromuscular junction and the locomotor defects seen in homozygous mir-12412 third instar larvae.

      (Sun et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      mir-12412
      (Sun et al., 2012)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)