- Tools
- Downloads
- Links
- Community
- About
- Help
FB2026_01
,
released March 12, 2026
A modified orb2 genomic fragment has been inserted into the attP site present in orb2attP via phiC31-mediated recombination. The resulting allele contains an insertion of a single nucleotide in the A isoform specific exon, and a deletion of a nucleotide from an exon common to orb2 isoforms A and B. These mutations restore the isoform A reading frame (which is tagged at the C-terminal end with GFP) whilst disrupting that of orb2B. A single mFRT11 site is present downstream of the orb2 coding sequence, and single attL and attR sites (generated during the recombination process) are also present.
orb2ΔA.T:Avic\GFP/orb2ΔB.T:Avic\GFP male flies have a long term 24 hour memory comparable to wild type when exposed to a courtship conditioning paradigm. Males that experience futile courtship of mated and therefore unreceptive females suppress their courtship towards other mated females.
orb2ΔQΔA.T:Avic\GFP/orb2ΔB.T:Avic\GFP male flies show defects in long term 24 hour memory when exposed to a courtship conditioning paradigm, and these are similar in severity to orb2ΔB.T:Avic\GFP/orb2ΔA.T:Avic\GFP males. Unlike wild type flies, males that experience futile courtship of mated and therefore unreceptive females often fail to suppress their courtship towards other mated females.
orb2::Mmus\Cpeb2RBD.GFP, orb2ΔBGFP has abnormal learning | adult stage phenotype
orb2::Mmus\Cpeb2RBD.T:Avic\GFP/orb2ΔB.T:Avic\GFP male flies have almost no long term 24 hour memory when exposed to a courtship conditioning paradigm. Unlike wild type flies, males that experience futile courtship of mated and therefore unreceptive females fail to suppress their courtship towards other mated females. Short term 1 hour memory is comparable to wild type.