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General Information
Symbol
Dmel\kukUASp.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Brandt
FlyBase ID
FBal0280959
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp regulatory sequences drive expression of a kuk cDNA (LD09231).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of kukScer\UAS.P\T.cBa in the longitudinal muscle cells of one week old flies under the control of Scer\GAL4Mhc.Switch.PO (induced by feeding RU486 to flies at the adult stages) results in a significant increase in nuclear perimeter and wrinkled, lobulated nuclei. Analysis using electron microscopy shows that these flies display nuclear phenotypes usually only observed during aging. Loss of peripheral heterochromatin, separation of inner and outer nuclear membrane, accumulation of ring like structures and a strong increase of dark material in the nucleoplasm are all observed. No changes in nuclear morphology are seen when kukScer\UAS.P\T.cBa is expressed in the fat body under the control of Scer\GAL4Switch1.106.

Expression of kukScer\UAS.P\T.cBa in adult muscle cells under the control of Scer\GAL4Mhc.Switch.PO (induced by feeding RU486 to flies at the adult stages) results in a 60% reduction in mean lifespan compared to controls. While the mortality rate is similar to controls, the time of onset differs. Expression of kukScer\UAS.P\T.cBa in either the abdominal fat body or the nervous system under the control of Scer\GAL4Switch1.106 and Scer\GAL4elav.Switch.PO respectively has no effect on lifespan.

The nuclei of fat body cells expressing kukScer\UAS.P\T.cBa under the control of Scer\GAL4Switch1.106 (induced by feeding RU486 to flies at the adult stages) are similar in size to controls. A small number of neurons show an increase in nuclear size when kukScer\UAS.P\T.cBa is expressed under the control of Scer\GAL4elav.Switch.PO.

Flies expressing kukScer\UAS.P\T.cBa under the control of Scer\GAL4Mhc.Switch.PO (induced by feeding RU486 to flies at the adult stages) show an early decline in climbing ability compared to controls. No climbing defects are seen when kukScer\UAS.P\T.cBa is expressed under the control of Scer\GAL4Switch1.106.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
kukScer\UAS.P\T.cBa
kukUASp.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Brandt
Secondary FlyBase IDs
    References (1)