FlyBase Allele Report: Scer\GAL4[Mhc.Switch.PO]

General Information

Symbol

Scer\GAL4^{Mhc.Switch.PO}

Species

S. cerevisiae

Name

FlyBase ID

FBal0128082

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Mhc-Switch.O}

Also Known As

MHC-GS-GAL4, MHC-GS, MHCGS, MHC-Geneswitch, MHC-GeneSwitch-Gal4, GS-MHC

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Osterwalder et al., 2001, Osterwalder et al., 2001)

Progenitor genotype

Carried in construct

P{Mhc-Switch.O}

Cytology

Description

A2.4kb fragment of the Mhc gene (including the promoter intron 1 and the 5'UTR exons 1 and 2) promoter drives expression of the ligand-inducible GAL4::GS driver.

(Osterwalder et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

Mhc

Mhc_MHC-GeneSwitch

Encoded product / tool

GAL4::GS

binary expression system - small molecule-regulated driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

somatic muscle cell

(Osterwalder et al., 2001)

first instar larval stage -- wandering third instar larval stage

somatic muscle cell

(Osterwalder et al., 2001)

adult stage

indirect flight muscle cell

(Poirier et al., 2008)

muscle cell of leg muscle of leg

(Poirier et al., 2008)

adult somatic muscle cell of abdomen

(Poirier et al., 2008)

adult digestive system

(Poirier et al., 2008)

adult muscle system

(Poirier et al., 2008)

Additional Information

Statement

Reference

Drives mifepristone-inducible expression in larval somatic muscle.

(FlyBase Curators, 2017)

ScerGAL4^{Mhc.Switch.PO} drives mifepristone-inducible expression in flight, leg, abdominal, labial, and digestive system muscles, and uninduced expression in indirect flight and leg muscles. It also exhibits uninduced expression in larval and pupal muscles.

(Poirier et al., 2008)

Marker for

larval somatic muscle cell

(Osterwalder et al., 2001)

somatic muscle cell

(Osterwalder et al., 2001)

Reflects expression of

Mhc

(Poirier et al., 2008, Osterwalder et al., 2001, Osterwalder et al., 2001)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal adult locomotory behavior, with Idit^UAS.Tag:FLAG

(Cobb et al., 2023)

abnormal adult locomotory behavior | recessive, with Idit^JF02930

(Cobb et al., 2023)

abnormal locomotor behavior | adult stage, with kuk^UASp.cBa

(Brandt et al., 2008)

abnormal locomotor behavior | adult stage, with Lam^UAS.cGa

(Brandt et al., 2008)

abnormal locomotor behavior | RU486 conditional, with Sh^{DN.EKI.UAS.GFP(GL)}

(Mosca et al., 2005)

abnormal neuroanatomy, with Cdc42^N17.UAS

(Nahm et al., 2010)

abnormal neuroanatomy, with Cdc42^V12.UAS

(Nahm et al., 2010)

abnormal neuroanatomy, with eIF4E1^UAS.cRa

(Menon et al., 2004)

abnormal neuroanatomy | third instar larval stage | RU486 conditional, with gbb^UAS.cUa

(Kim et al., 2019)

abnormal neurophysiology, with Sh^{EKO.UAS.GFP(GL)}

(Osterwalder et al., 2001)

abnormal neurophysiology | RU486 conditional, with Sh^{DN.EKI.UAS.GFP(GL)}

(Mosca et al., 2005)

abnormal neurophysiology | RU486 conditional, with Sh^{EKO.UAS.GFP(GL)}

(Osterwalder et al., 2001)

increased rate of movement | RU486 conditional, with Sh^{DN.EKI.UAS.GFP(GL)}

(Mosca et al., 2005)

lethal, with pum^UAS.fl

(Menon et al., 2004)

long lived | RU486 conditional, with AMPKα^WT.UAS

(Stenesen et al., 2013)

short lived, with kuk^UASp.cBa

(Brandt et al., 2008)

short lived, with Lam^UAS.cGa

(Brandt et al., 2008)

short lived | RU486 conditional

(Shen et al., 2009)

short lived | RU486 conditional, with AMPKα^KK102684

(Stenesen et al., 2013)

uncoordinated | RU486 conditional, with Sh^{DN.EKI.UAS.GFP(GL)}

(Mosca et al., 2005)

viable | RU486 conditional, with Sh^{DN.EKI.UAS.GFP(GL)}

(Mosca et al., 2005)

Phenotype Manifest In

bouton, with Cdc42^N17.UAS

(Nahm et al., 2010)

bouton, with Cdc42^V12.UAS

(Nahm et al., 2010)

embryonic/larval neuromuscular junction, with eIF4E1^UAS.cRa

(Menon et al., 2004)

embryonic/larval neuromuscular junction | third instar larval stage | RU486 conditional, with gbb^UAS.cUa

(Kim et al., 2019)

longitudinal muscle cell, with kuk^UASp.cBa

(Brandt et al., 2008)

longitudinal muscle cell, with Lam^UAS.cGa

(Brandt et al., 2008)

neuromuscular junction, with Cdc42^N17.UAS

(Nahm et al., 2010)

neuromuscular junction, with Cdc42^V12.UAS

(Nahm et al., 2010)

NMJ bouton | third instar larval stage | RU486 conditional, with gbb^UAS.cUa

(Kim et al., 2019)

nucleus | adult stage, with kuk^UASp.cBa

(Brandt et al., 2008)

nucleus | adult stage, with Lam^UAS.cGa

(Brandt et al., 2008)

type I bouton, with eIF4E1^UAS.cRa

(Menon et al., 2004)

visceral muscle fiber | larval stage | RU486 conditional, with Sh^{DN.EKI.UAS.GFP(GL)}

(Mosca et al., 2005)

Detailed Description

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO} adult flies show a significant, RU486 dependent, decrease in lifespan in both males and females.

(Shen et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl

(Menon et al., 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Suppressor of

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}/Lsd-2^HMS00629 is a suppressor | RU486 conditional of short lived | nutrition conditional phenotype of HDAC6^KO

(Yan et al., 2017)

Scer\GAL4^{Mhc.Switch.PO}/mask^HMS01045 is a suppressor | partially of abnormal flight phenotype of park^Δ21/park²⁵

(Zhu et al., 2015)

Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa

(Menon et al., 2004)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa has type I bouton phenotype, suppressible by Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa has embryonic/larval neuromuscular junction phenotype, suppressible by Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa has NMJ bouton phenotype, suppressible by Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl

(Menon et al., 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl has NMJ bouton phenotype, non-suppressible by Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl has type I bouton phenotype, non-suppressible by Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl has embryonic/larval neuromuscular junction phenotype, non-suppressible by Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Suppressor of

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}/mask^HMS01045 is a suppressor | partially of indirect flight muscle cell phenotype of park^Δ21/park²⁵

(Zhu et al., 2015)

Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl is a suppressor of type I bouton phenotype of Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl is a suppressor of embryonic/larval neuromuscular junction phenotype of Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl is a suppressor of NMJ bouton phenotype of Scer\GAL4^{Mhc.Switch.PO}, eIF4E1^UAS.cRa

(Menon et al., 2004)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl is a non-suppressor of NMJ bouton phenotype of Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl is a non-suppressor of type I bouton phenotype of Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl is a non-suppressor of embryonic/larval neuromuscular junction phenotype of Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl

(Menon et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^{Mhc.Switch.PO}/pum^UAS.fl fails to rescue pum⁹/pum⁷

(Menon et al., 2004)

Scer\GAL4^elav-C155, Scer\GAL4^{Mhc.Switch.PO}, pum^UAS.fl fails to rescue pum⁹/pum⁷

(Menon et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The "GeneSwitch" Scer\GAL4 protein produced by Scer\GAL4^{Mhc.Switch.PO} allows conditional expression of UAS transgenes, dependant on the presence of the activator RU486 (RU486 is thought to activate the protein produced by Scer\GAL4^{Mhc.Switch.PO} from a transcriptionally inactive to a transcriptionally active form). Expression of UAS transgenes by the Scer\GAL4^{Mhc.Switch.PO} protein is dose-dependent on RU486 concentration, with low background in the absence of RU486.

(Osterwalder et al., 2001)

The Scer\GAL4^{Mhc.Switch.PO} driver is leaky during the larval and pupal stages (driving expression of a UAS transgene in the absence of RU486).

(Poirier et al., 2008)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

MHCGS

(Lee and Schwarz, 2016)

Scer\GAL4^{Mhc.Switch.PO}

Name Synonyms

Secondary FlyBase IDs

References (41)

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