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General Information
Symbol
Dmel\ShDN.EKI.UAS.GFP(GL)
Species
D. melanogaster
Name
FlyBase ID
FBal0288215
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Sh(DN), EKI
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a dominant-negative form of Sh.

All Sh sequence 3' to the S1 transmembrane helix (residue 246 and C-wards) have been deleted from ShEKO.UAS.GFP(GL).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

When ShSDN.Scer\UAS.T:Avic\GFP-GL is driven by Scer\GAL4Mhc.Switch.PO or Scer\GAL4elav.Switch.PO, and the animals exposed to RU486, no significant reduction in viability is seen. When ShSDN.Scer\UAS.T:Avic\GFP-GL is driven by Scer\GAL4Mhc.Switch.PO , and the animals exposed to RU486, the voltage-activated transient K+ current (IA) in the larval muscle fibres is potently suppressed. IK is unaffected. The frequency of spontaneous synaptic activity in ShSDN.Scer\UAS.T:Avic\GFP-GL, Scer\GAL4elav.Switch.PO mutants exposed to RU486, is nearly twice that of wild-type. Excitatory postsynaptic potential (EPSP) duration is increased by ~49% when Scer\GAL4elav.Switch.PO is driven by Scer\GAL4elav.Switch.PO and exposed to RU486. When driven by Scer\GAL4Mhc.Switch.PO, a 37% increase is seen. EPSP amplitude is unaffected. Mutant adults exhibit both leg shaking and wing scissoring under ether anaesthetization, when driven by Scer\GAL4Mhc.Switch.PO or Scer\GAL4Mhc.Switch.PO and exposed to RU486. This behaviour persists in severed legs. When ShSDN.Scer\UAS.T:Avic\GFP-GL is driven by Scer\GAL4Mhc.Switch.PO mutant larvae (also exposed to RU486) crawl more slowly than wild-type. This affect is not seen if driven by Scer\GAL4elav.Switch.PO. When ShSDN.Scer\UAS.T:Avic\GFP-GL is driven by Scer\GAL4elav.Switch.PO or Scer\GAL4Mhc.Switch.PO and mutants are exposed to RU486 no effect is seen on arborisation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Other
Phenotype Manifest In
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of eagDN.EKI.Scer\UAS and ShDN.EKI.Scer\UAS.T:Avic\GFP-GL in the RP2 motor neurons (using the Scer\GAL4eve.RRK driver to drive expression of Scer\FLP1Scer\UAS.cUa which then induces clones of cells expressing Scer\GAL4Act.PU) results in a significant increase in the number of dendritic branches and increased dendritic complexity near the neuronal soma.

Co-expression of ShDN.EKI.Scer\UAS and eagDN.EKI.Scer\UAS under the control of Scer\GAL4D42 in the presence of Scer\GAL80Cha.PK (to suppress expression in cholinergic neurons) results in a significant increase in total dendritic length and in the number of dendritic branches in MN5 motorneurons.

The reduction in total dendritic length and in the number of dendritic branches which is seen in MN5 motorneurons in animals expressing JraJbz.Scer\UAS under the control of Scer\GAL4D42 in the presence of Scer\GAL80Cha.PK (to suppress expression in cholinergic neurons) is not altered if the flies are also simultaneously co-expressing both ShDN.EKI.Scer\UAS and eagDN.EKI.Scer\UAS.

Co-expression of both ShDN.EKI.Scer\UAS and eagDN.EKI.Scer\UAS under the control of Scer\GAL4futsch-C380 in the presence of Scer\GAL80Cha.PK (to suppress expression in all cholinergic neurons) results in the MN5 neuron showing a tonic firing response to a current injection into the soma. Resting membrane potential and input resistance of the MN5 neuron are unaffected.

The dendritic structure of the MN5 neuron is altered compared to wild type in animals expressing ShDN.EKI.Scer\UAS and eagDN.EKI.Scer\UAS under the control of Scer\GAL4futsch-C380. Total dendritic length, the number of branch points and the the mean length of individual dendritic branches is increased. Maximum branch order is unaffected. The mean distance of all dendritic segments to the origin of the tree and the total dendritic surface are increased.

Flies expressing both ShDN.EKI.Scer\UAS and eagDN.EKI.Scer\UAS under the control of Scer\GAL4futsch-C380 in the presence of Scer\GAL80Cha.PK show increased flight motor performance in a restrained flight assay. The initial and total flight time are significantly increased compared to wild type.

Motor neurons co-expressing eagDN.EKI.Scer\UAS and ShDN.EKI.Scer\UAS.T:Avic\GFP-GL under the control of Scer\GAL4futsch-C380 (in the presence of Scer\GAL80Cha.PK) have similar action potential thresholds as control neurons in response to a depolarising current, but have a significantly higher action potential frequency in response to a positive current. The postspike hyperpolarisation is smaller in the mutant larvae, often resulting in spike doublets.

Larval RP2 motor neurons co-expressing eagDN.EKI.Scer\UAS and ShDN.EKI.Scer\UAS.T:Avic\GFP-GL under the control of Scer\GAL4unspecified show a significant increase in dendrite volume compared to controls.

Cultured motor neurons co-expressing eagDN.EKI.Scer\UAS and ShDN.EKI.Scer\UAS.T:Avic\GFP-GL under the control of Scer\GAL4futsch-C380 (in the presence of Scer\GAL80Cha.PK to suppress expression in cholinergic neurons) show a significant increase in both neurite length and branch number compared to controls.

When ShSDN.Scer\UAS.T:Avic\GFP-GL is driven by Scer\GAL4Mhc.Switch.PO in a eag1 and mutants are exposed to RU486 no effect is seen on arborisation. If driven by Scer\GAL4elav.Switch.PO in a eag1 background synaptic arbor growth is significantly increased. The mean bouton count in NMJ boutons is almost doubled in these mutants.

Xenogenetic Interactions
Statement
Reference

Simultaneous co-expression of both eagDN.EKI.Scer\UAS and ShDN.EKI.Scer\UAS.T:Avic\GFP-GL enhances the progressive reduction in electroretinogram (ERG) amplitude which is seen in flies expressing Hsap\LRRK2G2019S.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4ple.PG, such that the triple mutant flies show a significant reduction in ERG amplitude at 10 days of age.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
ShDN.EKI.Scer\UAS.T:Avic\GFP-GL
ShDN.EKI.Scer\UAS
ShDN.EKI.UAS.GFP(GL)
ShSDN.Scer\UAS.T:Avic\GFP-GL
Name Synonyms
Secondary FlyBase IDs
  • FBal0282703
  • FBal0189960
References (7)