FlyBase Recombinant Construct Report: P{Mhc-Switch.O}

General Information

Symbol

P{Mhc-Switch.O}

FlyBase ID

FBtp0015154

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^{Mhc.Switch.PO}

Also Known As

MHC-GS-GAL4, MHC-GS, MHC-Geneswitch, MHC-GeneSwitch-Gal4, MHCGS, GS-MHC

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

3 available

Molecular map

Description and Uses

Construct components

Component allele Scer\GAL4^{Mhc.Switch.PO}

Transgenic product class

Component

Use(s)

Regulatory region(s)

Mhc

Mhc_MHC-GeneSwitch

Encoded product / tool

GAL4::GS

binary expression system - small molecule-regulated driver

Description

A2.4kb fragment of the Mhc gene (including the promoter intron 1 and the 5'UTR exons 1 and 2) promoter drives expression of the ligand-inducible GAL4::GS driver.

(Osterwalder et al., 2001)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^{Mhc.Switch.PO}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

somatic muscle cell

(Osterwalder et al., 2001)

first instar larval stage -- wandering third instar larval stage

somatic muscle cell

(Osterwalder et al., 2001)

adult stage

indirect flight muscle cell

(Poirier et al., 2008)

muscle cell of leg muscle of leg

(Poirier et al., 2008)

adult somatic muscle cell of abdomen

(Poirier et al., 2008)

adult digestive system

(Poirier et al., 2008)

adult muscle system

(Poirier et al., 2008)

Additional Information

Statement

Reference

Drives mifepristone-inducible expression in larval somatic muscle.

(FlyBase Curators, 2017)

ScerGAL4^{Mhc.Switch.PO} drives mifepristone-inducible expression in flight, leg, abdominal, labial, and digestive system muscles, and uninduced expression in indirect flight and leg muscles. It also exhibits uninduced expression in larval and pupal muscles.

(Poirier et al., 2008)

Marker for

larval somatic muscle cell

(Osterwalder et al., 2001)

somatic muscle cell

(Osterwalder et al., 2001)

Reflects expression of

Mhc

(Poirier et al., 2008, Osterwalder et al., 2001, Osterwalder et al., 2001)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

construct_comment: Drives expression of the "GeneSwitch" protein, an RU486-activated GAL4 fusion protein.

(Osterwalder et al., 2001)

construct_comment: Scer\ GAL4::Hsap \ PGR::Hsap \RELA fusion (Scer\GAL4^{Mhc.Switch.PO}) protein function induced upon application of RU486 ligand.See report for T:Hsap\PGR; see report for T:Hsap\RELA.

(Osterwalder et al., 2001)

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (22)

Reported As

Symbol Synonym

GS MHC

(Brandt et al., 2008)

GS-MHC

(Brandt et al., 2023, Brandt et al., 2008)

GS-MHC-Gal4

(Zhu et al., 2015)

MHC Gene-Switch

(Poirier et al., 2008)

MHC Geneswitch

(Barwell et al., 2017)

MHC-GAL4^Geneswitch

(Veverytsa and Allan, 2011)

MHC-GS

(Lennicke et al., 2025, Weigelt et al., 2020, Ashley et al., 2019, Ragheb et al., 2017, Carrillo et al., 2015, Shen et al., 2009)

MHC-GS-GAL4

(Yan et al., 2017, Stenesen et al., 2013, Nahm et al., 2010, Menon et al., 2004, Sherwood et al., 2004)

MHC-GS-Gal4

(Cobb et al., 2023, Damschroder et al., 2022, Kim et al., 2020)

MHC-GSGal4

(Wang et al., 2003)

MHC-GeneSwitch

(Ashley et al., 2019, Bauer et al., 2005, Bauer et al., 2005)

MHC-GeneSwitch-GAL4

(Nahm et al., 2010, Menon et al., 2009)

MHC-GeneSwitch-Gal4

(Fridell et al., 2005)

MHC-Geneswitch

(Lee and Schwarz, 2016)

MHC:GS

(Shen et al., 2009)

MHCGS

(Zhang et al., 2024, Martínez Corrales et al., 2020)

Mhc-GS-GAL4

(Kim et al., 2021, Kim et al., 2019)

Mhc-GS-Gal4

(Hunt and Demontis, 2022, Robles-Murguia et al., 2019)

P{Mhc-Switch.O}

(FlyBase, 1996-)

P{Switch-MHC}-gal4

(Banerjee et al., 2012)

mhcGS

(Bahhir et al., 2019)

pP{MHC-GeneSwitch}

(Osterwalder et al., 2001, Osterwalder et al., 2001)

Secondary FlyBase IDs

References (41)

Report Sections

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