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General Information
Symbol
Scer\GAL4Mhc.Switch.PO
Species
S. cerevisiae
Name
FlyBase ID
FBal0128082
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
MHC-GS-GAL4, MHC-GS, MHCGS, MHC-Geneswitch, MHC-GeneSwitch-Gal4
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A2.4kb fragment of the Mhc gene (including the promoter intron 1 and the 5'UTR exons 1 and 2) promoter drives expression of the ligand-inducible GAL4::GS driver.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives mifepristone-inducible expression in larval somatic muscle.

Scer\GAL4Mhc.Switch.PO drives mifepristone-inducible expression in flight, leg, abdominal, labial, and digestive system muscles, and uninduced expression in indirect flight and leg muscles. It also exhibits uninduced expression in larval and pupal muscles.

 
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4Mhc.Switch.PO adult flies show a significant, RU486 dependent, decrease in lifespan in both males and females.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Suppressed by
NOT suppressed by
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

The "GeneSwitch" Scer\GAL4 protein produced by Scer\GAL4Mhc.Switch.PO allows conditional expression of UAS transgenes, dependant on the presence of the activator RU486 (RU486 is thought to activate the protein produced by Scer\GAL4Mhc.Switch.PO from a transcriptionally inactive to a transcriptionally active form). Expression of UAS transgenes by the Scer\GAL4Mhc.Switch.PO protein is dose-dependent on RU486 concentration, with low background in the absence of RU486.

The Scer\GAL4Mhc.Switch.PO driver is leaky during the larval and pupal stages (driving expression of a UAS transgene in the absence of RU486).

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4Mhc.Switch.PO
Name Synonyms
Secondary FlyBase IDs
    References (33)