K949term | pum-PA; K601term | pum-PB; K949term | pum-PC; K949term | pum-PD; K351term | pum-PE; K341term | pum-PF; K949term | pum-PG; K337term | pum-PH
Nucleotide substitution: A3890T.
Mutation truncates the protein before the RNA-binding domain.
Amino acid replacement: ?949term.
Expression of pumScer\UAS.fl at 18oC in postmitotic neurons (Scer\GAL4elav-C155) rescues the synaptic morphology phenotypes found in a pum9/pum7 background. Both the average size and the number of type 1b boutons on muscle 12 are restored to wild-type.
Expression of pumScer\UAS.fl in muscles (Scer\GAL4Mhc.Switch.PO) in pum9/pum7 larvae reduces the number of eIF-4E aggregates by 7-fold, to 3.5 aggregates per neuromuscular junction. The level of eIF-4E is reduced by approximately 6.5-fold. In contrast, neural expression of pumScer\UAS.fl (Scer\GAL4elav-C155) in pum9/pum7 larvae does not greatly decrease the number of eIF-4E aggregates (although a 20% reduction is seen). Levels of eIF-4E are 88% of those in pum9/pum7 larvae.
The increase in 1s bouton number in pum9/pum7 mutants is fully rescued through expression of pumScer\UAS.fl in muscles (Scer\GAL4Mhc.Switch.PO), and is unaffected by expression in neurons (under the control of Scer\GAL4elav-C155).
The mEJP amplitude and quantal content phenotypes of pum9/pum7 mutants are not rescued to near wild-type levels by neuronal (Scer\GAL4elav-C155), muscle (Scer\GAL4Mhc.Switch.PO), or dual expression of pumScer\UAS.fl.
Expression of pumRBD.Scer\UAS at 18oC in postmitotic neurons (Scer\GAL4elav-C155) does not rescue the morphology phenotypes found in a pum9/pum7 background, in contrast to its ability to provide the early abdominal segmentation function.