FlyBase Allele Report: Dmel\Arfip[71]

General Information

Symbol

Dmel\Arfip⁷¹

Species

D. melanogaster

Name

FlyBase ID

FBal0281697

Feature type

allele

Associated gene

Dmel\Arfip

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Chang et al., 2013)

amorphic allele - molecular evidence

(Chang et al., 2013, Peru Y Colón de Portugal et al., 2012)

Mutagen

Delta2-3 transposase

(Chang et al., 2013)

Progenitor genotype

P{EPgy2}Arfip^EY11874

(Chang et al., 2013)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion which removes part of Arfip and part of the neighbouring CG17721 gene.

(Chang et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage (with Arfip¹²)

(Chang et al., 2015)

Golgi apparatus (with Arfip¹²)

(Chang et al., 2013)

NMJ bouton | second instar larval stage (with Arfip¹²)

(Chang et al., 2013)

NMJ bouton | third instar larval stage (with Arfip¹²)

(Chang et al., 2015, Chang et al., 2013)

NMJ bouton | third instar larval stage (with Df(3R)ED5518)

(Chang et al., 2013)

Detailed Description

Statement

Reference

Arfip¹²/Arfip⁷¹ third instar larvae have significantly fewer boutons at the neuromuscular junction (muscle 4) compared to Arfip¹²/+ heterozygotes. Arfip¹²/Arfip⁷¹ larval NMJs are similar to wild type in terms ofRMP, mEJP, EJP and quantal content.

(Chang et al., 2015)

Newly eclosed homozygous escapers have no obvious neurological phenotypes and are able to fly and climb walls.

The average number of boutons at the neuromuscular junction is significantly reduced compared to controls in Arfip⁷¹/Df(3R)ED5518 and Arfip⁷¹/Arfip¹² third instar larvae. The average number of boutons at the neuromuscular junction is also reduced in Arfip⁷¹/Arfip¹² second instar larvae.

Axonal transport is normal in Arfip⁷¹/Arfip¹² third instar larvae.

The Golgi appears smaller and more fragmented than normal in the ventral nerve cord of Arfip⁷¹/Arfip¹² third instar larvae.

(Chang et al., 2013)

Arfip¹²/Arfip⁷¹ flies are sensitive to ethanol-induced sedation compared to wild-type controls.

(Peru Y Colón de Portugal et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Arfip⁷¹/Arfip¹² has abnormal neuroanatomy | third instar larval stage phenotype, non-enhanceable by garz[+]/garz^Δ137

(Chang et al., 2015)

Other

Statement

Reference

Arfip⁷¹, garz[+]/garz^Δ137 has abnormal neuroanatomy | third instar larval stage phenotype

(Chang et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Arfip⁷¹/Arfip¹² has NMJ bouton | third instar larval stage phenotype, non-enhanceable by garz[+]/garz^Δ137

(Chang et al., 2015)

Arfip⁷¹/Arfip¹² has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by garz[+]/garz^Δ137

(Chang et al., 2015)

Other

Statement

Reference

Arfip⁷¹, garz[+]/garz^Δ137 has NMJ bouton | third instar larval stage phenotype

(Chang et al., 2015)

Arfip⁷¹, garz[+]/garz^Δ137 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Chang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

garz^Δ137/+;Arfip⁷¹/+ or garz^Δ137/+;Arfip¹²/+ third instar larvae have significantly fewer boutons at the neuromuscular junction compared to Arfip⁷¹/+ heterozygotes. garz^Δ137/+ does not enhance the reduction in bouton number seen in Arfip⁷¹/Arfip¹² larvae.

Sec5^E10/+;Arfip⁷¹/+ or Sec15¹/+;Arfip⁷¹/+ third instar larvae do not show a reduction in bouton number at the neuromuscular junction, compared to Sec5^E10/+ or Sec15¹/+ heterozygotes.

(Chang et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Arfip⁷¹/Arfip¹² is rescued by Arfip^UAS.WT/Scer\GAL4^RapGAP1-OK6

(Chang et al., 2015)

Arfip⁷¹/Arfip¹² is rescued by Scer\GAL4^elav-C155/Arfip^UAS.WT

(Chang et al., 2013)

Arfip⁷¹/Arfip¹² is rescued by Arfip^UAS.WT/Scer\GAL4^RapGAP1-OK6

(Chang et al., 2013)

Arfip⁷¹/Arfip¹² is rescued by Arfip^UAS.Tag:HA/Scer\GAL4^hs.PU

(Peru Y Colón de Portugal et al., 2012)

Not rescued by

Arfip⁷¹/Arfip¹² is not rescued by Arfip^{GBD.UAS.Tag:HA}/Scer\GAL4^RapGAP1-OK6

(Chang et al., 2015)

Arfip⁷¹/Arfip¹² is not rescued by Scer\GAL4^elav-C155/Arfip^{GBD.UAS.Tag:HA}

(Chang et al., 2015)

Arfip⁷¹/Arfip¹² is not rescued by Scer\GAL4^elav-C155/Arfip^{BAR.UAS.Tag:HA}

(Chang et al., 2013)

Arfip⁷¹/Arfip¹² is not rescued by Scer\GAL4^RapGAP1-OK6/Arfip^{BAR.UAS.Tag:HA}

(Chang et al., 2013)

Comments

Expression of Arfip^Scer\UAS.WT driven by Scer\GAL4^RapGAP1-OK6 (motor-neuron specific) rescues the reduced bouton number seen at the neuromuscular junction of Arfip¹²/Arfip⁷¹ third instar larvae. Expression of Arfip^{GBD.Scer\UAS.T:Ivir\HA1} by either Scer\GAL4^elav-C155 (pan-neuronal) or Scer\GAL4^RapGAP1-OK6 does not rescue the reduced bouton number seen at the neuromuscular junction of Arfip¹²/Arfip⁷¹ third instar larvae.

(Chang et al., 2015)

Expression of Arfip^Scer\UAS.WT under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^Rapgap1-OK6 rescues the reduction in bouton number at the neuromuscular junction seen in Arfip⁷¹/Arfip¹² third instar larvae.

Expression of Arfip^{BAR.Scer\UAS.T:Ivir\HA1} under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^Rapgap1-OK6 fails to rescue the reduction in bouton number at the neuromuscular junction seen in Arfip⁷¹/Arfip¹² third instar larvae.

(Chang et al., 2013)

Expression of Arfip^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^hs.PU at 29[o]C rescues the sensitivity of Arfip¹²/Arfip⁷¹ flies to ethanol-induced sedation.

(Peru Y Colón de Portugal et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

79624

w¹¹¹⁸; Kr^If-1/CyO; Arfip⁷¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Arfip⁷¹

(Peru Y Colón de Portugal et al., 2012)

arfip⁷¹

(Chang et al., 2015, Chang et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (3)

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