Recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them, including part of Gfrl.
Scer\FRT-mediated recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them, including part of Gfrl.
Gfrldel larvae are frequently immobile or sluggish in response to touch and show a range of feeding defects, as compared to controls: a foraging phenotype in which larvae move away from a central food source; accumulation of food in the oesophagus; reduced/absence of food in the midguts and absence of food in the oesophagus. Embryos show asymmetry of the frontal ganglion and disruption to the esophageal ganglia.
Gfrldel/Df(3R)Exel6185 females mated to wild-type males show a reduction of approximately 60% in absolute fertility and a reduction of approximately in fecundity (measured as number of progeny per female at 72 hours post-mating).
Gfrldel/+ females mated to wild-type females have fertility comparable to that of wild-type females.
Homozygous and Gfrldel/Df(3R)Exel6185 females lay very few eggs. Most of the eggs laid are small (showing a significant reduction in length compared to wild type) and translucent, and have dorsal appendage defects and do not develop into larvae.
Homozygous and Gfrldel/Df(3R)Exel6185 males show significantly reduced fertility and homozygous males show significantly reduced fecundity compared to wild type.
Gfrldel/Df(3R)Exel6185 has male semi-sterile phenotype, enhanceable by Fas2e76
Gfrldel has male semi-sterile | recessive phenotype, enhanceable by Fas2e76
Fas2e76, Gfrldel has male sterile phenotype
Gfrldel/Df(3R)Exel6185, Fas2e76 has male sterile phenotype
Gfrldel/Df(3R)BSC518, Fas2e76 has male sterile phenotype
Fas2e76 ; Gfrldel/Gfrldel, Fas2e76 ; Gfrldel/Df(3R)Exel6185 and Fas2e76 ; Gfrldel/Df(3R)BSC518 double mutant males are completely infertile.
Gfrldel is rescued by Scer\GAL4da.G32/GfrlUAS.A.Tag:V5
Expression of GfrlScer\UAS.A.T:SV5\V5 under the control of Scer\GAL4da.G32 rescues the morphology defect of eggs laid by homozygous Gfrldel females.