FlyBase Allele Report: Hsap\MAPT[UAS.S2A]

General Information

Symbol

Hsap\MAPT^UAS.S2A

Species

H. sapiens

Name

FlyBase ID

FBal0281834

Feature type

allele

Associated gene

Hsap\MAPT

Associated Insertion(s)

Carried in Construct

P{UAS-Tau.S2A}

Also Known As

UAS-Tau^S2A, UAS-htau^2N4RS2A

Key Links

Transgenic product class

missense_variant

(Chatterjee et al., 2009)

polypeptide_post_translational_processing_variant

(Chatterjee et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Chatterjee et al., 2009)

polypeptide_post_translational_processing_variant

(Chatterjee et al., 2009)

Mutagen

in vitro construct

(Chatterjee et al., 2009)

Progenitor genotype

P{UAS-Tau.S2A}

(Chatterjee et al., 2009)

Carried in construct

P{UAS-Tau.S2A}

Cytology

Description

Serines 262 and 356 of P{UAS-Tau.S2A}, the two main par-1 phosphorylation sites, are transformed to alanines.

(Chatterjee et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\MAPT

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of tauopathy

CEA

(Papanikolopoulou et al., 2010)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

interommatidial bristle, with Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

retina, with Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Detailed Description

Statement

Reference

Expression of Hsap\MAPT^Scer\UAS.S2A under the control of Scer\GAL4^Bx-MS1096 does not affect mitosis in third instar larval wing disc cells. Expression under the Scer\GAL4^GMR.PU does not cause any adult eye phenotype and neither does it exacerbate the phenotype (missing interommatidial bristles) caused by the expression of Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG}.

(Bougé and Parmentier, 2016)

Pan-neuronal expression of Hsap\MAPT^Scer\UAS.S2A under the control of Scer\GAL4^elav.PU does not affect mushroom body morphology.

(Kosmidis et al., 2010)

Flies expressing Hsap\MAPT^Scer\UAS.S2A in neurons under the control of Scer\GAL4^elav.PU do not show mushroom body aberrations.

Adult onset expression of Hsap\MAPT^Scer\UAS.S2A under the control of Scer\GAL4^elav.PU and Scer\GAL80^ts.αTub84B has no significant effect on the associative olfactory learning performance of the animals compared with controls. The performance of animals expressing Hsap\MAPT^Scer\UAS.S2A continuously throughout development is also not different from controls.

(Papanikolopoulou et al., 2010)

Adult eyes expressing Hsap\MAPT^Scer\UAS.S2A under the control of Scer\GAL4^GMR.PS appear relatively normal with only mild bristle abnormalities. Retinal sections reveal only a modest effect on retinal morphology.

(Chatterjee et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\MAPT^UAS.S2A, Scer\GAL4^GMR.PS has interommatidial bristle phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.S2A, Scer\GAL4^GMR.PS has eye phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.S2A, Scer\GAL4^GMR.PS has retina phenotype, enhanceable by par-1^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

NOT Enhanced by

Statement

Reference

Hsap\MAPT^UAS.S2A, Scer\GAL4^GMR.PS has interommatidial bristle phenotype, non-enhanceable by sgg^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.S2A, Scer\GAL4^GMR.PS has eye phenotype, non-enhanceable by sgg^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Hsap\MAPT^UAS.S2A, Scer\GAL4^GMR.PS has retina phenotype, non-enhanceable by sgg^UAS.cSa, Scer\GAL4^GMR.PS

(Chatterjee et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

HnRNP-K^KG02524 does not induce a rough eye phenotype in flies expressing Hsap\MAPT^Scer\UAS.S2A under the control of Scer\GAL4^GMR.PS.

CaMKI^EY07197 does not induce a rough eye phenotype in flies expressing Hsap\MAPT^Scer\UAS.S2A under the control of Scer\GAL4^GMR.PS.

Hop^k14504 does not induce a rough eye phenotype in flies expressing Hsap\MAPT^Scer\UAS.S2A under the control of Scer\GAL4^GMR.PS.

Klp61F⁰⁷⁰¹² does not induce a rough eye phenotype in flies expressing Hsap\MAPT^Scer\UAS.S2A under the control of Scer\GAL4^GMR.PS.

par-1^k06323 does not induce a rough eye phenotype in flies expressing Hsap\MAPT^Scer\UAS.S2A under the control of Scer\GAL4^GMR.PS.

(Ambegaokar and Jackson, 2011)

Coexpression of par-1^Scer\UAS.cSa results in only a modest worsening of the Hsap\MAPT^Scer\UAS.S2A, Scer\GAL4^GMR.PS rough-eye phenotype; the double transgenics have slightly more irregular eyes with disordered and occasional missing bristles. The internal retinal morphology of flies coexpressing par-1^Scer\UAS.cSa and Hsap\MAPT^Scer\UAS.S2A is only slightly worse than that of flies expressing Hsap\MAPT^Scer\UAS.S2A alone, with slightly misshapen rhabdomeres and a moderate loss of photoreceptor neurons.

The relatively normal eye phenotype of Hsap\MAPT^Scer\UAS.S2A, Scer\GAL4^GMR.PS flies remains unchanged when sgg^Scer\UAS.cSa is co-expressed.

(Chatterjee et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

51365

w¹¹¹⁸; P{UAS-Tau.S2A}1.62

51364

y¹ w¹¹¹⁸; P{UAS-Tau.S2A}1.33

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\MAPT^Scer\UAS.S2A

Hsap\MAPT^UAS.S2A

Name Synonyms

Secondary FlyBase IDs

References (8)

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