UASp regulatory sequences drive expression of full-length Chro which is tagged at the N-terminal end with a TAP tag (3xTag:HA, 3xTag:FLAG) and at the C-terminal end with GFP.
Scer\GAL4unspecified/ChroUASp.FL.Tag:HA,Tag:FLAG,GFP rescues Chro71/Chro612
Expression of ChroScer\UAS.P\T.FL.T:Ivir\HA1,T:Zzzz\FLAG,T:Avic\GFP in third larval instar salivary glands rescues all aspects of polytene chromosome morphology in Chro71/Chro612 animals.