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General Information
Symbol
Dmel\Chro612
Species
D. melanogaster
Name
FlyBase ID
FBal0243233
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Truncated Chro612 protein is missing parts of the COOH-terminal domain important for spindle localisation.

EMS-generated point mutation in Chro that introduces a premature stop codon resulting in a truncated 612 amino acid protein that retains the chromodomain.

Nucleotide substitution: C2024T

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In contrast to wild-type, in Chro71/Chro612 mutant larvae at 96h after larval hatching (ALH) 26% of mira-positive central brain neural stem cells (NSCs) retain their cellular processes and number of actively dividing dpn-positive NSCs is much reduced compared to controls. Chro612/Df(3L)ED231 mutants display similar NSC reactivation defects.

Polytene chromosomes of Chro71/Chro612 third instar larvae show disruptions of the band/interband regions and the chromosome arms are coiled and condensed.

90% of Chro71/Chro612 mutant larval neuroblasts have severe spindle and chromosome segregation defects. The microtubule spindles are incomplete, unfocussed, and/or without clear spindle poles. At anaphase, chromosomes are lagging and scattered.

The mitotic index is significantly reduced in Chro71/Chro612 mutants, and an associated small brain and imaginal disc phenotype is observed.

Chro71/Chro612 transheterozygotes survive to third instar larval stage but no pupae are observed.

Polytene chromosome morphology is disturbed in Chro71/Chro612 mutant third instar larvae.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The central brain neuroblast reactivation defects (manifested by the retention of cellular extensions and low mitotic activity even at late larval stages) characteristic for Chro71/Chro612 mutants are alleviated by expression of grhScer\UAS.cUa under the control of Scer\GAL4insc-Mz1407 in the mutant background or by combination with a single copy of pros17, the phenotype can be further improved when both grhScer\UAS.cUa expression as well as the pros17 heterozygosity are combined.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The brain neuroblast reactivation defects observed in either Chro71/Chro612 or Chro612/Df(3L)ED231 mutant larvae at 96h after larval hatching can be rescued by combination with Chro+tCH322-159M1.

Expression of ChroScer\UAS.P\T.FL.T:Ivir\HA1,T:Zzzz\FLAG,T:Avic\GFP in third larval instar salivary glands rescues all aspects of polytene chromosome morphology in Chro71/Chro612 animals.

Expression of ChroNTD.Scer\UAS.P\T.T:Avic\GFP.T:SV40\nls2 in third larval instar salivary glands substantially rescues polytene chromosome morphology in Chro71/Chro612 animals, although some regions of the chromosome arms remain coiled.

Expression of either ChroNTD-ΔChD.Scer\UAS.T:Uuuu\nls6,T:SV5\V5 or ChroChD.Scer\UAS.T:SV40\nls2,T:SV5\V5,T:Avic\GFP in third larval instar salivary glands results in no or very little improvement of polytene chromosome morphology in Chro71/Chro612 animals.

Expression of ChroCTD.Scer\UAS.P\T.T:Avic\GFP in third larval instar salivary glands does not rescue polytene chromosome morphology in Chro71/Chro612 animals.

Expression of ChroScer\UAS.P\T.T:Avic\GFP with Scer\GAL4elav-C155 partially rescues the smaller brain size, and coiled and condensed chromosome arm morphology of the polytene chromosomes from Chro71/Chro612 mutant larvae.

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Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)