UASt regulatory sequences drive expression of amino acid residues 1-219 of Chro, which are tagged at the C-terminal end with 3 copies of a nuclear localization signal and with Tag:V5.
Scer\GAL4unspecified/ChroNTD-ΔChD.UAS.Tag:NLS(Unk),Tag:V5 fails to rescue Chro71/Chro612
Expression of ChroNTD-ΔChD.Scer\UAS.T:Uuuu\nls6,T:SV5\V5 in third larval instar salivary glands results in no or very little improvement of polytene chromosome morphology in Chro71/Chro612 animals.