UASt regulatory sequences drive expression of amino acid residues 219-277 of Chro (the chromodomain), which are tagged at the C-terminal end with a nuclear localization signal (3 copies of Tag:NLS(SV40-largeT)), Tag:V5 and GFP.
Expression of ChroChD.Scer\UAS.T:SV40\nls2,T:SV5\V5,T:Avic\GFP in third larval instar salivary glands results in no or very little improvement of polytene chromosome morphology in Chro71/Chro612 animals.