FlyBase Allele Report: Dmel\pyd[RNAi.3.UAS]

General Information

Symbol

Dmel\pyd^RNAi.3.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0282549

Feature type

allele

Associated gene

Dmel\pyd

Associated Insertion(s)

Carried in Construct

P{UAS-pyd.RNAi.3}

Key Links

Transgenic product class

RNAi_reagent

(Seppa et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Seppa et al., 2008)

Mutagen

in vitro construct

(Seppa et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-pyd.RNAi.3}

Cytology

Description

UAS regulatory sequences drive expression of an inverse repeat targeting a region common to four out of five predicted isoforms of pyd. The 519bp fragment begins 2662bp after the start codon of pyd-RB.

(Seppa et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

pyd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | pupal stage, with Scer\GAL4^GMR.PF

(Seppa et al., 2008)

increased cell number | pupal stage, with Scer\GAL4^GMR.PF

(Seppa et al., 2008)

visible | adult stage, with Scer\GAL4^GMR.PF

(Seppa et al., 2008)

Phenotype Manifest In

cone cell | pupal stage, with Scer\GAL4^GMR.PF

(Seppa et al., 2008)

eye, with Scer\GAL4^GMR.PF

(Seppa et al., 2008)

interommatidial precursor cell | pupal stage | increased number, with Scer\GAL4^GMR.PF

(Seppa et al., 2008)

primary pigment cell | pupal stage, with Scer\GAL4^GMR.PF

(Seppa et al., 2008)

tertiary pigment cell | pupal stage | increased number, with Scer\GAL4^GMR.PF

(Seppa et al., 2008)

Detailed Description

Statement

Reference

Adult flies expressing pyd^{3.dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF exhibit ectopic thoracic bristles and a mildly rough eye. Inter-ommatidial precursor cell (IPC) patterning errors are observed at 41-42 hours after pupal formation (APF). A mild increase is seen in the total number of IPCs surrounding each ommatidium, especially around the bristles. The IPCs are poorly placed, resulting in breakdown of the hexagonal pattern to pentagonal or squared ommatidial arrays and the integrity of the 1[o]/1[o] interface is occasionally disrupted by an IPC that makes direct contact with the cone cells (referred to as cone-contact cells). Three cells are observed to share the 3[o] cell niche equally, where normally a single cell is found in this position. Similar defects are observed when pyd^{3.dsRNA.Scer\UAS} is expressed predominantly in IPCs under the control of Scer\GAL4⁵⁴. Defects in IPC patterning are also seen when pyd^{3.dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF at 20h APF. As in wild type, local movements are seen but these are undirected and unproductive and the cells often fail to undergo intercalation to create single cell rows.

The number of cone cells in the ommatidia of flies expressing pyd^{3.dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF is occasionally altered, and incorrect contacts are often seen within a cone cell quartet. 8.1% of mutant flies fail to undergo the change from having anterior/posterior (A/P) apical contacts to the equatorial/polar (E/P) configuration that occurs in wild type ommatidia between 22 and 28h APF, with a subset of cone cells maintaining the immature A/P arrangement through to 42h APF.

Expression of pyd^{3.dsRNA.Scer\UAS} under the control of Scer\GAL4^sd-SG29.1 has no visible effect on the wing veins.

(Seppa et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, pyd^RNAi.3.UAS has increased cell number | pupal stage phenotype, enhanceable by shg[+]/shg^R69

(Seppa et al., 2008)

Scer\GAL4^GMR.PF, pyd^RNAi.3.UAS has increased cell number | pupal stage phenotype, enhanceable by rst⁶/rst[+]

(Seppa et al., 2008)

Scer\GAL4^GMR.PF, pyd^RNAi.3.UAS has increased cell number | pupal stage phenotype, enhanceable by tkv⁸/tkv[+]

(Seppa et al., 2008)

Enhancer of

Statement

Reference

Scer\GAL4^sd-SG29.1/pyd^RNAi.3.UAS is an enhancer of visible | adult stage phenotype of Scer\GAL4^sd-SG29.1, tkv^RNAi.IR2.UAS

(Seppa et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, pyd^RNAi.3.UAS has interommatidial precursor cell | pupal stage | increased number phenotype, enhanceable by shg[+]/shg^R69

(Seppa et al., 2008)

Scer\GAL4^GMR.PF, pyd^RNAi.3.UAS has interommatidial precursor cell | pupal stage | increased number phenotype, enhanceable by rst⁶/rst[+]

(Seppa et al., 2008)

Scer\GAL4^GMR.PF, pyd^RNAi.3.UAS has interommatidial precursor cell | pupal stage | increased number phenotype, enhanceable by tkv⁸/tkv[+]

(Seppa et al., 2008)

Scer\GAL4^GMR.PF, pyd^RNAi.3.UAS has cone cell | pupal stage phenotype, enhanceable by shg^UAS.sgGFP/Scer\GAL4^GMR.PF

(Seppa et al., 2008)

Scer\GAL4^GMR.PF, pyd^RNAi.3.UAS has cone cell | pupal stage phenotype, enhanceable by α-Cat^UAS.sgGFP, Scer\GAL4^GMR.PF

(Seppa et al., 2008)

Enhancer of

Statement

Reference

Scer\GAL4^sd-SG29.1/pyd^RNAi.3.UAS is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^sd-SG29.1, tkv^RNAi.IR2.UAS

(Seppa et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of shg^R69 strongly enhances the increase in inter-ommatidial precursor cell number seen when pyd^{3.dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

One copy of rst⁶ strongly enhances the increase in inter-ommatidial precursor cell number seen when pyd^{3.dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

One copy of tkv⁸ strongly enhances the increase in inter-ommatidial precursor cell number seen when pyd^{3.dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of pyd^{3.dsRNA.Scer\UAS} strongly enhances the wing vein defects seen when tkv^{dsRNA.IR2.Scer\UAS} is expressed under the control of Scer\GAL4^sd-SG29.1.

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} strongly enhances the cone cell switching phenotype seen 41-42 hours after pupal formation when pyd^{3.dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF. A greater proportion of ommatidia maintain the immature A/P configuration rather than switching to the E/P configuration as is seen in controls.

Expression of α-Cat^{Scer\UAS.T:Avic\GFP-sg} strongly enhances the cone cell switching phenotype seen 41-42 hours after pupal formation when pyd^{3.dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF. A greater proportion of ommatidia maintain the immature A/P configuration rather than switching to the E/P configuration as is seen in controls.

(Seppa et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pyd^{3.dsRNA.Scer\UAS}

pyd^3.dsRNA.UAS

pyd^RNAi.3.UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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