FlyBase Allele Report: Dmel\α-Cat[UAS.sgGFP]

General Information

Symbol

Dmel\α-Cat^UAS.sgGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0095916

Feature type

allele

Associated gene

Dmel\α-Cat

Associated Insertion(s)

Carried in Construct

P{UAS-α-Cat.T:GFP.sg}

Also Known As

UAS-α-catenin-GFP, α-catenin-GFP, UAS-DαC-GFP, UAS-α-Cat-GFP, UAS-Dα-cat-GFP, UAS-Dα-catenin-GFP, UAS-αCat-GFP, α-Cat-GFP

Key Links

Transgenic product class

wild_type

(Oda and Tsukita, 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Oda and Tsukita, 1999)

Mutagen

in vitro construct

(Oda and Tsukita, 1999)

Progenitor genotype

Carried in construct

P{UAS-α-Cat.T:GFP.sg}

Cytology

Description

UAS regulatory sequences drive expression of full length α-Cat tagged at the carboxyl terminus with sgGFP.

(Oda and Tsukita, 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

α-Cat

Tagged with

sgGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa

(Collins et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Larson et al., 2008)

Phenotype Manifest In

cone cell, with Scer\GAL4^GMR.PF

(Seppa et al., 2008)

Detailed Description

Statement

Reference

Expression of Scer\GAL4^GMR.PF driven α-Cat^{Scer\UAS.T:Avic\GFP-sg} does not effect patterning of the eye compared to Scer\GAL4^GMR.PF alone.

(Larson et al., 2008)

Expression of α-Cat^{Scer\UAS.T:Avic\GFP-sg} under the control of Scer\GAL4^GMR.PF results in low level failure to switch cone-cell contacts.

(Seppa et al., 2008)

α-Cat^{Scer\UAS.T:Avic\GFP-sg} when driven by Scer\GAL4^btl.PS produces embryos with normal adherens junction patterns. Almost all (98.9%) dorsal trunk fusions are successful.

(Oda and Tsukita, 1999)

No morphological abnormalities are seen in embryos expressing α-Cat^{Scer\UAS.T:Avic\GFP-sg} under the control of Scer\GAL4^en-e16E.

(Oda and Tsukita, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

α-Cat^UAS.sgGFP/Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy phenotype of rst⁶

(Larson et al., 2008)

α-Cat^UAS.sgGFP/Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy phenotype of p120ctn³⁰⁸

(Larson et al., 2008)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PU, α-Cat^UAS.sgGFP is a suppressor | partially of visible | adult stage phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Collins et al., 2023)

SREBP^GAL4::VP16/α-Cat^UAS.sgGFP is a suppressor | partially of abnormal cell size | larval stage phenotype of Axn¹²⁷

(Zhang et al., 2017)

SREBP^GAL4::VP16/α-Cat^UAS.sgGFP is a suppressor of visible | larval stage phenotype of Axn¹²⁷

(Zhang et al., 2017)

α-Cat^UAS.sgGFP/Scer\GAL4^GMR.PF is a suppressor of abnormal neuroanatomy phenotype of N^fa-g

(Larson et al., 2008)

Phenotype Manifest In

Enhancer of

Statement

Reference

α-Cat^UAS.sgGFP, Scer\GAL4^GMR.PF is an enhancer of cone cell | pupal stage phenotype of Scer\GAL4^GMR.PF, pyd^RNAi.3.UAS

(Seppa et al., 2008)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PU, α-Cat^UAS.sgGFP is a suppressor | partially of eye phenotype of HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, Scer\GAL4^GMR.PU

(Collins et al., 2023)

SREBP^GAL4::VP16/α-Cat^UAS.sgGFP is a suppressor of larval abdomen phenotype of Axn¹²⁷

(Zhang et al., 2017)

SREBP^GAL4::VP16/α-Cat^UAS.sgGFP is a suppressor | partially of larval fat cell phenotype of Axn¹²⁷

(Zhang et al., 2017)

Other

Statement

Reference

α-Cat^UAS.sgGFP, Df(2R)E2/shg², Scer\GAL4^btl.PS has larval tracheal system phenotype

(Oda and Tsukita, 1999)

α-Cat^UAS.sgGFP, Df(2R)E2/shg², Scer\GAL4^btl.PS has embryonic/larval tracheal dorsal trunk phenotype

(Oda and Tsukita, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Scer\GAL4^GMR.PF driven α-Cat^{Scer\UAS.T:Avic\GFP-sg} leads to a mild suppression of the N^fa-g pupal retina phenotypes.

Expression of Scer\GAL4^GMR.PF driven α-Cat^{Scer\UAS.T:Avic\GFP-sg} leads to a mild enhancement of some of the rst⁶ pupal retina patterning phenotypes.

Expression of Scer\GAL4^GMR.PF driven α-Cat^{Scer\UAS.T:Avic\GFP-sg} leads to a mild enhancement of the p120ctn³⁰⁸ pupal retina patterning error phenotypes.

(Larson et al., 2008)

Expression of α-Cat^{Scer\UAS.T:Avic\GFP-sg} strongly enhances the cone cell switching phenotype seen 41-42 hours after pupal formation when pyd^{3.dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF. A greater proportion of ommatidia maintain the immature A/P configuration rather than switching to the E/P configuration as is seen in controls.

(Seppa et al., 2008)

shg²/Df(2R)E2 embryos with α-Cat^{Scer\UAS.T:Avic\GFP-sg} driven by Scer\GAL4^btl.PS, show largely disordered tracheal branching. No successful dorsal trunk fusions are seen in these embryos. The addition of shg^Scer\UAS.cOa to these flies rescues these phenotypes to nearly wild-type. Tracheal patterning is normal, although tracheal branches and connectives in the embryos are less extended than in wild-type.

(Oda and Tsukita, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

58787