FlyBase Allele Report: Dmel\tll[RNAi.UAS]

General Information

Symbol

Dmel\tll^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0284096

Feature type

allele

Associated gene

Dmel\tll

Associated Insertion(s)

Carried in Construct

P{UAS-tll.RNAi}

Also Known As

TLL miRNA, UAS-tll-miRNA[s]

Key Links

Transgenic product class

RNAi_reagent

(Lin et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Lin et al., 2009)

Mutagen

in vitro construct

(Lin et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-tll.RNAi}

Cytology

Description

UAS drives expression of a transcript encoding two copies of mir-6-1 modified to target tll.

(Lin et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tll

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^ey-OK107

(Lin et al., 2009)

adult optic lobe | pharate adult stage, with Scer\GAL4^C855a

(Guillermin et al., 2015)

lamina | pharate adult stage, with Scer\GAL4^C855a

(Guillermin et al., 2015)

lamina forming neuroblast | larval stage, with Scer\GAL4^C855a

(Guillermin et al., 2015)

lamina forming neuroblast | larval stage | somatic clone, with Scer\GAL4^C855a

(Guillermin et al., 2015)

lamina furrow | larval stage, with Scer\GAL4^C855a

(Guillermin et al., 2015)

larval inner optic anlage, with Scer\GAL4^C855a

(Guillermin et al., 2015)

larval optic anlage, with Scer\GAL4^C855a

(Guillermin et al., 2015)

larval outer optic anlage, with Scer\GAL4^C855a

(Guillermin et al., 2015)

larval outer optic anlage | somatic clone, with Scer\GAL4^Tub.PU

(Guillermin et al., 2015)

medulla | pharate adult stage, with Scer\GAL4^C855a

(Guillermin et al., 2015)

mushroom body | adult stage, with Scer\GAL4^ey-OK107

(Lin et al., 2009)

optic lobe neuroepithelial cell | somatic clone, with Scer\GAL4^Tub.PU

(Guillermin et al., 2015)

Detailed Description

Statement

Reference

Expression of tll^{miRNA.Scer\UAS} under the control of Scer\GAL4^{CG13917-BG01278} has no significant effect on male fertility (number of progeny produced by wild-type females mated to the knock-down males).

(Misra et al., 2017)

The expression of tll^miRNA.UAS under the control of Scer\GAL4^C855a leads to significantly smaller larval and pharate adult optic lobes (including inner and outer proliferating centers, lamina and medulla) and all pharate optic ganglia apart from the lamina cannot be distinguished from each other; the larval optic lobe also present severely disrupted lamina columns with severely decreased numbers of lamina precursor cells (identified by Dac immunostaining) and a reduced or completely absent lamina furrow. Clonal expression under the control of Scer\GAL4^Tub.PU results in clonal cells showing an increase in apoptosis (cleaved Casp-3) and being extruded from the neuroepithelium to become aberrantly localized in the medulla cortex.

(Guillermin et al., 2015)

Expression using Scer\GAL4^ey-OK107 results in 100% of mushroom bodies having only a few neurons left.

(Lin et al., 2009)

Scer\GAL4^ey-OK107-mediated expression of tll^{miRNA.Scer\UAS} affects mushroom body proliferation, resulting in a tiny adult mushroom body purely consisting of early-type mushroom body neurons.

(Lin et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Tub.PU, tll^RNAi.UAS has increased cell death | somatic clone | larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Tub.PU

(Guillermin et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Tub.PU, tll^RNAi.UAS has larval outer optic anlage | somatic clone phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Tub.PU

(Guillermin et al., 2015)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^Tub.PU, tll^RNAi.UAS has larval outer optic anlage | somatic clone phenotype

(Guillermin et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The co-expression of BacA\p35^UAS.cHa suppresses the increased apoptosis induced by the clonal expression of tll^miRNA.UAS under the control of Scer\GAL4^Tub.PU, leading to large clones that remain in the neuroepithelium but show abnormal epithelial morphology, namely ectopic folds; neuroepithelial cells in these clones can transform into neuroblasts, as expected in controls.

(Guillermin et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

tll^RNAi.UAS

tll^{miRNA.Scer\UAS}

tll^miRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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