FlyBase Allele Report: Maur\Prp[UAS.cFa]

General Information

Symbol

Maur\Prp^UAS.cFa

Species

M. auratus

Name

Saccharomyces cerevisiae UAS construct a of Fernandez-Funez

FlyBase ID

FBal0284419

Feature type

allele

Associated gene

Maur\Prp

Associated Insertion(s)

Carried in Construct

P{UAS-HaPrp.F}

Transgenic product class

wild_type

(Fernandez-Funez et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Fernandez-Funez et al., 2009)

Mutagen

in vitro construct

(Fernandez-Funez et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-HaPrp.F}

Cytology

Description

UASt drives expression of the open reading frame of wild type Maur\Prp.

(Fernandez-Funez et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Maur\Prp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of prion disease

CEA

(Myers et al., 2022, Zhang et al., 2014, Fernandez-Funez et al., 2010, Fernandez-Funez et al., 2009)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of prion disease

is ameliorated by Hsap\HSPA1L^UAS.cWa

(Fernandez-Funez et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^BG380

(Fernandez-Funez et al., 2010)

abnormal locomotor behavior | adult stage, with Scer\GAL4^BG380

(Zhang et al., 2014)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^Act.PU

(Fernandez-Funez et al., 2009)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^BG380

(Fernandez-Funez et al., 2009)

abnormal locomotor behavior | adult stage | progressive | heat sensitive, with Scer\GAL4^BG380, Scer\GAL80^ts.αTub84B

(Fernandez-Funez et al., 2009)

abnormal neuroanatomy | adult stage | progressive, with Scer\GAL4^da.G32

(Fernandez-Funez et al., 2009)

abnormal neuroanatomy | adult stage | progressive, with Scer\GAL4^ey-OK107

(Fernandez-Funez et al., 2009)

abnormal neuroanatomy | progressive, with Scer\GAL4^da.G32

(Fernandez-Funez et al., 2010)

abnormal neuroanatomy | progressive, with Scer\GAL4^ey-OK107

(Fernandez-Funez et al., 2010)

viable, with Scer\GAL4^elav.PU

(Myers et al., 2022)

Phenotype Manifest In

adult brain, with Scer\GAL4^da.G32

(Fernandez-Funez et al., 2010)

adult brain | progressive, with Scer\GAL4^da.G32

(Fernandez-Funez et al., 2009)

adult brain cell body rind, with Scer\GAL4^da.G32

(Fernandez-Funez et al., 2010)

adult brain cell body rind | progressive, with Scer\GAL4^da.G32

(Fernandez-Funez et al., 2009)

adult mushroom body alpha-lobe, with Scer\GAL4^ey-OK107

(Fernandez-Funez et al., 2010)

adult mushroom body alpha-lobe | progressive, with Scer\GAL4^ey-OK107

(Fernandez-Funez et al., 2009)

adult optic lobe, with Scer\GAL4^da.G32

(Fernandez-Funez et al., 2010)

adult optic lobe | adult stage | progressive, with Scer\GAL4^da.G32

(Fernandez-Funez et al., 2009)

Kenyon cell, with Scer\GAL4^ey-OK107

(Fernandez-Funez et al., 2010)

neuropil, with Scer\GAL4^da.G32

(Fernandez-Funez et al., 2010)

neuropil | adult stage | progressive, with Scer\GAL4^da.G32

(Fernandez-Funez et al., 2009)

Detailed Description

Statement

Reference

Adult flies expressing Maur\Prp^Scer\UAS.cFa under the control of Scer\GAL4^BG380 display locomotor dysfunction (impaired climbing ability).

(Zhang et al., 2014)

The brains of young flies expressing Scer\GAL4^da.G32>Maur\Prp^Scer\UAS.cFa are anatomically normal. These flies show well preserved architecture of the cortex, which contains the cell bodies of the brain neurons and the neuropil, the projections that occupy the center of the brain. In contrast, the brains of 30-day-old flies expressing Scer\GAL4^da.G32>Maur\Prp^Scer\UAS.cFa are smaller (reduced surface) and contain abundant vacuoles in the neuropil of the brain and optic lobes. At higher magnification, the cortex appears very thin and contain numerous micro-vacuoles, suggesting significant neuronal loss.

The mushroom body has normal aspect in young flies expressing Scer\GAL4^ey-OK107>Maur\Prp^Scer\UAS.cFa, indicating normal mushroom body development. Expression of Scer\GAL4^ey-OK107>Maur\Prp^Scer\UAS.cFa is highly neurotoxic and results in the progressive degeneration of the mushroom body alpha lobes. The dynamic destruction of these projections can be seen by the active retraction of axonal terminals (blebbing).

The size of the Kenyon cell clusters in young flies expressing Maur\Prp^Scer\UAS.cFa under the control of Scer\GAL4^ey-OK107 is comparable to controls, indicating normal Kenyon cell development. Older flies expressing Scer\GAL4^ey-OK107>Maur\Prp^Scer\UAS.cFa display a dramatic reduction in cluster size, indicating significant cell loss.

Flies expressing Maur\Prp^Scer\UAS.cFa under the control of Scer\GAL4^BG380 show severe locomotor dysfunction by the age of 3 days. 20-day-old flies expressing Scer\GAL4^BG380>Maur\Prp^Scer\UAS.cFa are less active than controls and most flies appear paralysed for long periods of time. The walking speed of flies expressing Scer\GAL4^BG380>Maur\Prp^Scer\UAS.cFa show a dramatic reduction compared with controls.

(Fernandez-Funez et al., 2010)

Scer\GAL4^BG380-mediated expression of Maur\Prp^Scer\UAS.cFa triggers a severe locomotor dysfunction (decreased performance in climbing assay) only 3 days after eclosion, and by day 6 no climbing ability is registered. A similar effect is observed if Maur\Prp^Scer\UAS.cFa expression is induced after eclosion (day 1) using the Scer\GAL80 system.

Expression of Maur\Prp^Scer\UAS.cFa in all brain neurons using Scer\GAL4^da.G32 results in normal brain architecture and cellular organization in 1 day old files. However, 30 day old flies display large holes in the brain and optic lobes - large and small vacuoles localize to the neuropile and the cortex. Additionally, the cortex is much thinner in the older flies, suggesting significant neuronal loss. The older fly brains also show cytosolic vacuolation, nuclear condensation and abnormal membrane folding.

40 day old, but not 1 day old, fly brains expressing Maur\Prp^Scer\UAS.cFa under the control of Scer\GAL4^ey-OK107 display prominent degeneration of mushroom body alpha lobes.

Scer\GAL4^Act.PU-mediated expression of Maur\Prp^Scer\UAS.cFa results in a steady decline in climbing ability over 20 days.

20 day old Scer\GAL4^Act.PU Maur\Prp^Scer\UAS.cFa flies show reduced speed and distribution when tracked in a custom arena.

(Fernandez-Funez et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Maur\Prp^UAS.cFa, Scer\GAL4^Act.PU has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible | partially by Hsap\HSPA1L^UAS.cWa, Scer\GAL4^Act.PU

(Fernandez-Funez et al., 2009)

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav.PU/Maur\Prp^UAS.cFa is a non-enhancer of visible | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Myers et al., 2022)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Maur\Prp^UAS.cFa is a non-suppressor of visible | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Myers et al., 2022)

Phenotype Manifest In

Suppressed by

Statement

Reference

Maur\Prp^UAS.cFa, Scer\GAL4^da.G32 has adult brain | progressive phenotype, suppressible | partially by Hsap\HSPA1L^UAS.cWa, Scer\GAL4^da.G32

(Fernandez-Funez et al., 2009)

Maur\Prp^UAS.cFa, Scer\GAL4^ey-OK107 has adult mushroom body alpha-lobe | progressive phenotype, suppressible | partially by Hsap\HSPA1L^UAS.cWa, Scer\GAL4^ey-OK107

(Fernandez-Funez et al., 2009)

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav.PU/Maur\Prp^UAS.cFa is a non-enhancer of eye phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Myers et al., 2022)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Maur\Prp^UAS.cFa is a non-suppressor of eye phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Myers et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

30 day old Maur\Prp^Scer\UAS.cFa Scer\GAL4^da.G32 fly brains co-expressing Hsap\HSPA1L^Scer\UAS.cWa exhibit very few vacuolated cells and normal nuclei.

40 day old Maur\Prp^Scer\UAS.cFa Scer\GAL4^ey-OK107 fly brains co-expressing Hsap\HSPA1L^Scer\UAS.cWa demonstrate robust protection of mushroom body projections.

Co-expression of Hsap\HSPA1L^Scer\UAS.cWa reduces the decline in climbing ability seen in Maur\Prp^Scer\UAS.cFa Scer\GAL4^Act.PU flies.

Co-expression of Hsap\HSPA1L^Scer\UAS.cWa suppresses the reduced distribution and speed seen in 20 day old Maur\Prp^Scer\UAS.cFa Scer\GAL4^Act.PU flies when tracked in a custom arena.

(Fernandez-Funez et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Maur\Prp^Scer\UAS.cFa

Maur\Prp^UAS.cFa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Fernandez-Funez

Secondary FlyBase IDs

References (5)

Report Sections

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