FlyBase Allele Report: Scer\GAL4[VGlut1.VGN9281]

General Information

Symbol

Scer\GAL4^{VGlut1.VGN9281}

Species

S. cerevisiae

Name

FlyBase ID

FBal0284792

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{VGlut1-GAL4.VGN9281}

Also Known As

VGN9281-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Brierley et al., 2009)

Progenitor genotype

Carried in construct

P{VGlut1-GAL4.VGN9281}

Cytology

Description

849bp of wild type DNA upstream (-3.8kb to -4.6kb) of the of VGlut translation start site regulates expression of a GAL4 driver.

(Brierley et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

VGlut1

VGlut1_VGN9281

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

pharate adult stage P15(i)

motor neuron | subset

(Brierley et al., 2009)

interneuron | subset

(Brierley et al., 2009)

adult stage

motor neuron | subset of tibia

(Syed et al., 2016)

Additional Information

Statement

Reference

ScerGAL4^{VGlut.VGN9281} drives expression in a single motor neuron of the tibia that innervates the tarsus reductor muscle.

(Syed et al., 2016)

ScerGAL4^{VGlut-VGN9281} drives expression in one late-born motorneuron and in a small number of interneurons.

(Brierley et al., 2009)

Marker for

Reflects expression of

VGlut1

(Brierley et al., 2009)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal copulation | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Sadaf et al., 2015)

abnormal courtship behavior | adult stage | male limited, with Hsap\KCNJ2^UAS.EGFP

(Sadaf et al., 2015)

abnormal neurophysiology | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Sadaf et al., 2015)

abnormal neurophysiology | adult stage | heat sensitive, with shi^1.UAS

(Sadaf et al., 2015)

flightless, with Hsap\KCNJ2^UAS.EGFP

(Sadaf et al., 2015)

flightless | heat sensitive, with shi^1.UAS

(Sadaf et al., 2015)

uncoordinated | adult stage | heat sensitive, with shi^1.UAS

(Sadaf et al., 2015)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Sadaf et al., 2015)

dorsal longitudinal indirect flight muscle cell | heat sensitive, with shi^1.UAS

(Sadaf et al., 2015)

wing | heat sensitive, with shi^1.UAS

(Sadaf et al., 2015)

Detailed Description

Statement

Reference

Inhibition (using shi^1.UAS at 29[o]C) of Scer\GAL4^{VGlut.VGN9281} cells in adults significantly reduces the number of animals capable of flight, compared to controls; animals that do not fly have significant wing coordination defects, compared to controls; there is also a significant reduction in the duration of electrophysiological spiking in dorsal longitudinal indirect flight muscles in response to an air puff, compared to controls.

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^{VGlut.VGN9281} cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, significantly reduces the number of animals capable of flight, compared to controls; there is also a significant reduction in the number of animals that display electrophysiological spiking in dorsal longitudinal indirect flight muscles in response to an air puff, compared to controls; courtship behaviors, including wing extension and wing vibration, and copulation success are severely impaired in males, compared to controls, when no Gal80[ts] transgene is used; none of these phenotypes occur when inhibition is restricted to a smaller set of cells using Scer\GAL80^tsh-GAL80.

(Sadaf et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Scer\GAL4^{VGlut-VGN9281}

Scer\GAL4^{VGlut.VGN9281}

Scer\GAL4^{VGlut1.VGN9281}

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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