FlyBase Allele Report: Dmel\sv[GMR.PS]

General Information

Symbol

Dmel\sv^GMR.PS

Species

D. melanogaster

Name

Glass Multimer Reporter promoter construct of Shi

FlyBase ID

FBal0284794

Feature type

allele

Associated gene

Dmel\sv

Associated Insertion(s)

Carried in Construct

P{GMR-sv.S}

Key Links

Transgenic product class

cDNA

(Shi and Noll, 2009)

wild_type

(Shi and Noll, 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Shi and Noll, 2009)

wild_type

(Shi and Noll, 2009)

Mutagen

in vitro construct

(Shi and Noll, 2009)

Progenitor genotype

Carried in construct

P{GMR-sv.S}

Cytology

Description

A 2.8 kb EcoRI-XbaI fragment obtained from the sv cDNA cpx1, which includes the entire ORF but none of the multiple ployA addition sites, is under the control of sev regulatory sequences.

(Shi and Noll, 2009)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cone cell

(Shi and Noll, 2009)

eye

(Shi and Noll, 2009)

eye photoreceptor cell

(Shi and Noll, 2009)

interommatidial precursor cell | pupal stage | increased number

(Shi and Noll, 2009)

Detailed Description

Statement

Reference

Flies carrying one copy of sv^GMR.PS show a strong rough eye phenotype - frequent loss of photoreceptor cells and their strong deformation is observed. In pupal sv^GMR.PS/+ eye discs, 78% of ommatidia possess the normal number of four cone cells, 13% have fewer, and only 9% have one supernumerary cone cell. A large number of supernumerary cells are observed between the ommatidia.

(Shi and Noll, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

sv^GMR.PS is a suppressor of increased cell death | late third instar larval stage phenotype of ttk^{88.GMR.Tag:MYC}

(Shi and Noll, 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

sv^GMR.PS has cone cell | pupal stage | increased number phenotype, enhanceable by ttk^{88.GMR.Tag:MYC}

(Shi and Noll, 2009)

Enhancer of

Statement

Reference

sv^GMR.PS is an enhancer of cone cell | pupal stage | increased number phenotype of ttk^{88.GMR.Tag:MYC}

(Shi and Noll, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Ectopic expression of both sv^GMR.PS and ttk^{88.GMR.T:Hsap\MYC} generates supernumerary cone cells in about 90% of the ommatidia of pupal eye discs, with a maximum of 11 cone cells observed in one ommatidium.

More than half of the ommatidia in sv^GMR.PS ttk^{88.GMR.T:Hsap\MYC} eyes have two or three supernumerary cone cells: 30% have three, 24% have two, and 14% have one supernumerary cone cell.

The addition of sv^GMR.PS results in less photoreceptor cell death in ttk^{88.GMR.T:Hsap\MYC} third instar eye discs.

(Shi and Noll, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

sv^GMR.PS

Name Synonyms

Glass Multimer Reporter promoter construct of Shi

Secondary FlyBase IDs

References (1)

Report Sections

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