FlyBase Allele Report: Dmel\GlyRS[EX34]

General Information

Symbol

Dmel\GlyRS^EX34

Species

D. melanogaster

Name

FlyBase ID

FBal0284914

Feature type

allele

Associated gene

Dmel\GlyRS

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Chihara et al., 2007)

Progenitor genotype

P{EPgy2}GlyRS^EY09021

(Chihara et al., 2007)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes two-thirds of the Aats-gly open reading frame.

(Chihara et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Charcot-Marie-Tooth disease type 2

CEA

(Chihara et al., 2007)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | somatic clone

(Chihara et al., 2007)

Phenotype Manifest In

adult antennal lobe projection neuron DL1 adPN | somatic clone

(Chihara et al., 2007)

gamma Kenyon cell | adult stage | somatic clone

(Chihara et al., 2007)

Detailed Description

Statement

Reference

Adult DL1 projection neuron homozygous single-cell clones lack dendritic and axonal terminal arborisations.

At 18 hours after puparium formation (APF), single-cell homozygous DL1 projection neuron clones elaborate dendritic terminals similar to those of wild type in the appropriate position of the proto-antennal lobe and axonal growth is only slightly shorter than normal. However, by 50 hours APF, the dendritic arborisation has completely disappeared and axonal terminals have not extended beyond that seen at 18 hours APF.

Homozygous neuroblast clones show reduced projection neuron cell numbers compared to control clones.

Adult mushroom body γ neuron single-cell homozygous clones show a significant reduction in the number of dendritic branches and dendritic claws compared to wild type. The number of terminal axonal branches is also severely reduced in the mutant cells.

(Chihara et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

GlyRS^EX34 has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible | somatic clone by Hsap\GARS1^UAS.Tag:MYC/Scer\GAL4^GH146

(Chihara et al., 2007)

GlyRS^EX34 has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible | partially | somatic clone by Scer\GAL4^GH146/Hsap\GARS1^{E71G.UAS.Tag:MYC}

(Chihara et al., 2007)

NOT suppressed by

Statement

Reference

GlyRS^EX34 has abnormal neuroanatomy | somatic clone | adult stage phenotype, non-suppressible | somatic clone by Hsap\GARS1^{L129P.UAS.Tag:MYC}/Scer\GAL4^GH146

(Chihara et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

GlyRS^EX34 has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, suppressible | somatic clone by Hsap\GARS1^UAS.Tag:MYC/Scer\GAL4^GH146

(Chihara et al., 2007)

GlyRS^EX34 has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, suppressible | partially | somatic clone by Scer\GAL4^GH146/Hsap\GARS1^{E71G.UAS.Tag:MYC}

(Chihara et al., 2007)

NOT suppressed by

Statement

Reference

GlyRS^EX34 has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype, non-suppressible | somatic clone by Hsap\GARS1^{L129P.UAS.Tag:MYC}/Scer\GAL4^GH146

(Chihara et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\GARS^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GH146 completely rescues the dendritic and axonal arborisation of homozygous Aats-gly^EX34 DL1 projection neurons.

Expression of Hsap\GARS^{E71G.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GH146 partially rescues the dendritic and axonal arborisation of homozygous Aats-gly^EX34 DL1 projection neurons: the rescued cells show sparse dendritic elaboration and loss of the dorsal axonal branch.

Expression of Hsap\GARS^{L129P.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GH146 fails to rescue the dendritic and axonal arborisation of homozygous Aats-gly^EX34 DL1 projection neurons.

(Chihara et al., 2007)

Complementation and Rescue Data

Rescued by

GlyRS^EX34 is rescued by GlyRS^UAS.Tag:MYC/Scer\GAL4^GH146

(Chihara et al., 2007)

Comments

Expression of Aats-gly^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GH146 fully rescues the mutant phenotypes of homozygous Aats-gly^EX34 DL1 projection neurons.

(Chihara et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Aats-gly^EX34

GlyRS^EX34

gars^EX34

(Chihara et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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