FlyBase Allele Report: Dmel\RhoGAP100F[CD]

General Information

Symbol

Dmel\RhoGAP100F^CD

Species

D. melanogaster

Name

FlyBase ID

FBal0284969

Feature type

allele

Associated gene

Dmel\RhoGAP100F

Associated Insertion(s)

Carried in Construct

Also Known As

syd-1^CD

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Holbrook et al., 2012)

Progenitor genotype

PBac{WH}FeCH^f02660

(Holbrook et al., 2012)

PBac{WH}RhoGAP100F^f04641

(Holbrook et al., 2012)

Caused by aberration

Df(3R)RhoGAP100F^CD

Cytology

Description

Scer\FRT-mediated recombination between the two progenitor insertions has resulted in deletion of the genomic sequence between them (deletion of 30884bp). This removes RhoGAP100F and most of FeCH.

(Holbrook et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:31,811,512..31,842,396 [+]

Comment:

Scer\FRT-mediated recombination between the two progenitor insertions has resulted in deletion of the genomic sequence between them (deletion of 30884bp). This removes RhoGAP100F and most of FeCH.

(Holbrook et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | somatic clone

(Holbrook et al., 2012)

abnormal neuroanatomy | adult stage | somatic clone (with RhoGAP100F^w46)

(Holbrook et al., 2012)

Phenotype Manifest In

photoreceptor cell R7 | somatic clone

(Holbrook et al., 2012)

photoreceptor cell R7 | somatic clone (with RhoGAP100F^w46)

(Holbrook et al., 2012)

Detailed Description

Statement

Reference

R7 axon terminals in RhoGAP100F^w46/RhoGAP100F^CD animals often fail to contact the M6 layer of the medulla. Some R7 axon terminals project thin extensions beyond M6.

Axon terminals of homozygous R7 photoreceptor cell clones often fail to contact the M6 layer of the medulla. Those axons that do contact M6 have abnormally small terminal boutons which often project thin extensions beyond M6. The mutant axon terminals are indistinguishable from wild type at 24 hours after puparium formation (APF), but at 55 hours APF the defect of R7 axons failing to contact M6 is maximal. The mutant R7 axons do not project extensions at 55 hours APF, but extensions are visible by 72 hours APF.

(Holbrook et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

RhoGAP100F^CD has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible | partially | somatic clone by Scer\GAL4^Act.PU/Liprin-α^UAS.Tag:HA

(Holbrook et al., 2012)

RhoGAP100F^CD has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible | partially | somatic clone by Scer\GAL4^Act.PU/trio^UAS.cBa

(Holbrook et al., 2012)

NOT suppressed by

Statement

Reference

RhoGAP100F^CD has abnormal neuroanatomy | somatic clone | adult stage phenotype, non-suppressible | somatic clone by Scer\GAL4^Act.PU/Liprin-β^UAS.Tag:HA

(Holbrook et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

RhoGAP100F^CD has photoreceptor cell R7 | somatic clone phenotype, suppressible | partially | somatic clone by Scer\GAL4^Act.PU/Liprin-α^UAS.Tag:HA

(Holbrook et al., 2012)

RhoGAP100F^CD has photoreceptor cell R7 | somatic clone phenotype, suppressible | partially | somatic clone by Scer\GAL4^Act.PU/trio^UAS.cBa

(Holbrook et al., 2012)

NOT suppressed by

Statement

Reference

RhoGAP100F^CD has photoreceptor cell R7 | somatic clone phenotype, non-suppressible | somatic clone by Scer\GAL4^Act.PU/Liprin-β^UAS.Tag:HA

(Holbrook et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Liprin-α^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU in RhoGAP100F^CD R7 photoreceptor cell clones partially suppresses the failure of the axon terminals to contact the M6 layer of the medulla. The later tendency for R7 axons that do reach the M6 layer to project extensions is not suppressed.

Expression of Liprin-β^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU in RhoGAP100F^CD R7 photoreceptor cell clones has no effect on either the failure of the axon terminals to contact the M6 layer of the medulla or the tendency for the R7 axons that do reach the M6 layer to project extensions.

Expression of trio^Scer\UAS.cBa under the control of Scer\GAL4^Act.PU in RhoGAP100F^CD R7 photoreceptor cell clones almost completely suppresses the failure of the axon terminals to contact the M6 layer of the medulla. The later tendency for R7 axons that do reach the M6 layer to project extensions is partially suppressed.

(Holbrook et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

RhoGAP100F^CD is rescued by Scer\GAL4^Act.PU/RhoGAP100F^UAS.Tag:FLAG

(Holbrook et al., 2012)

Comments

Expression of RhoGAP100F^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Act.PU rescues the axon terminal defects seen in RhoGAP100F^CD R7 photoreceptor cell clones.

(Holbrook et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

RhoGAP100F^CD

syd-1^CD

(Spinner et al., 2018, Holbrook et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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