FlyBase Allele Report: Dmel\nAChRα7[D197A.UAS]

General Information

Symbol

Dmel\nAChRα7^D197A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0285442

Feature type

allele

Associated gene

Dmel\nAChRα7

Associated Insertion(s)

Carried in Construct

P{UAS-nAChRα7.D197A}

Key Links

Transgenic product class

missense_variant

(Mejia et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Mejia et al., 2013)

Mutagen

in vitro construct

(Mejia et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-nAChRα7.D197A}

Cytology

Description

UASt regulatory sequences drive expression of nAChRα7 containing the amino acid replacement D197A.

(Mejia et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

nAChRα7

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^shot-OK307

(Mejia et al., 2013)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell, with Scer\GAL4^shot-OK307

(Mejia et al., 2013)

Detailed Description

Statement

Reference

Expression of gfA^{D197A.Scer\UAS} under the control of Scer\GAL4^OK307 has no effect on the refractory period of the response of the dorsal longitudinal muscle to stimulation of the giant fiber. However, the maximum following frequency is significantly reduced compared to wild type.

Expression of gfA^{D197A.Scer\UAS} under the control of Scer\GAL4^OK307 has no significant effect on the latency of the response of the tergotrochanteral muscle to stimulation of the giant fiber.

(Mejia et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

nAChRα7^D197A.UAS/Scer\GAL4^shot-OK307 partially rescues nAChRα7^PΔEY6

(Mejia et al., 2013)

Comments

Expression of gfA^{D197A.Scer\UAS} under the control of Scer\GAL4^OK307 rescues an electrophysiological response of the dorsal longitudinal muscle (DLM) to stimulation of the giant fiber. However, the DLMs are only able to follow 10 pulses given at 100Hz with 17.3% reliability and the maximum frequency to follow 10 stimuli (13 +/- 7 Hz) is severely reduced compared to wild type.

(Mejia et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

64146

w^*; P{UAS-nAChRα7.D197A}3

64145

w^*; P{UAS-nAChRα7.D197A}2/CyO

Notes on Origin

Discoverer