FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\EndoAS75D.CyPet
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General Information
Symbol
Dmel\EndoAS75D.CyPet
Species
D. melanogaster
Name
FlyBase ID
FBal0285577
Feature type
allele
Associated gene
Dmel\EndoA
Associated Insertion(s)
Carried in Construct
PBac{EndoA-CyPet.S75D}
Also Known As
endoAS75D
Key Links
Transgenic product class
missense_variant
(Matta et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Matta et al., 2012)
Mutagen
in vitro construct
(Matta et al., 2012)
Progenitor genotype
EndoACyPet
(Matta et al., 2012)
Carried in construct
PBac{EndoA-CyPet.S75D}
Cytology
Description

Amino acid replacement: S75D.

(Matta et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
EndoA
Encoded product / tool
EndoA
Tagged with
CyPet
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      EndoAΔ4/EndoA26, EndoAS75D.CyPet larvae show increased autophagosomes (Atg8-mCherry) and autolysosomes (Lamp-GFP) at NMJ boutons, under both fed and starved conditions.

      (Hernandez-Diaz et al., 2022)

      In EndoAΔ4/EndoA26 transheterozygotes containing a copy of EndoAS75D.T:Avic\GFP-CyPet, the presynaptic region of third instar larval neuromuscular junctions presents an abnormally high density of autophagosomes (detected as fluorescent puncta of Atg8 or Lamp in confocal microscopy images) under normal diet, but does not present defects in the starvation-induced autophagosome formation, as compared to controls. Adults exhibit significant decreases in the number of dopaminergic PPM3 and PPL1 neurons, but not dopaminergic PPM1 and PPM2 neurons, at day 30, but not day 1, after eclosion, as compared to controls. In adults reared under constant light, but not under constant dark, the eye exhibits a significant decrease in the rhabdomere number per ommatidium and in the photoreceptor terminal number per lamina cartridge (at day 7 of light exposure), as compared to controls; the eye of individuals reared under either constant light or constant dark also exhibits a significant decrease in depolarization amplitude in response to light pulses (at day 3 of light exposure), as compared to controls.

      (Soukup et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      EndoAS75D.CyPet is a suppressor of autophagosome | third instar larval stage | nutrition conditional phenotype of Lrrke03680

      (Soukup et al., 2016)
      NOT Suppressor of
      Statement
      Reference

      EndoA26, EndoAΔ4, EndoAS75D.CyPet is a non-suppressor of autophagosome | larval stage | decreased number | nutrition conditional phenotype of EndoB4

      (Hernandez-Diaz et al., 2022)

      EndoA26, EndoAΔ4, EndoAS75D.CyPet is a non-suppressor of embryonic/larval neuromuscular junction | larval stage | nutrition conditional phenotype of EndoB4

      (Hernandez-Diaz et al., 2022)

      EndoA26, EndoAΔ4, EndoAS75D.CyPet is a non-suppressor of NMJ bouton | larval stage | nutrition conditional phenotype of EndoB4

      (Hernandez-Diaz et al., 2022)

      EndoA26, EndoAΔ4, EndoAS75D.CyPet is a non-suppressor of autolysosome | larval stage | decreased number | nutrition conditional phenotype of EndoB4

      (Hernandez-Diaz et al., 2022)
      Other
      Statement
      Reference

      EndoAS75D.CyPet, Lrrke03680 has autophagosome | third instar larval stage | nutrition conditional phenotype

      (Soukup et al., 2016)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      One copy of EndoAS75D.T:Avic\GFP-CyPet rescues the failure in starvation-induced autophagosome formation observed at the presynaptic region of Lrrke03680-homozygous third instar larval neuromuscular junctions, but, under normal diet, leads to a significant increase in the density of autophagosomes at the same site.

      (Soukup et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      EndoAS75D.CyPet
      EndoAS75D.T:Avic\GFP-CyPet
      endoAS75D.T:Avic\GFP-CyPet
      endoAS75D
      (Hernandez-Diaz et al., 2022, Soukup et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)