FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\AxnK446E.αTub84B.Tag:FLAG
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General Information
Symbol
Dmel\AxnK446E.αTub84B.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0285789
Feature type
allele
Associated gene
Dmel\Axn
Associated Insertion(s)
Carried in Construct
P{αTub84B(-FRT)Axn.K446E.FLAG}
Key Links
Transgenic product class
missense_variant
(Kremer et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Kremer et al., 2010)
Mutagen
FLPase
(Kremer et al., 2010)
Progenitor genotype
AxnK446E.FRT.αTub84B.Tag:FLAG
(Kremer et al., 2010)
Carried in construct
P{αTub84B(-FRT)Axn.K446E.FLAG}
Cytology
Description

Excision of the FRT-w cassette from progenitor AxnK446E.FRT.αTub84B.Tag:FLAG allows the αTub84B promoter to drive expression of an K446E mutant of Axn tagged with N-terminal 6xTag:FLAG.

(Kremer et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
Axn
Tagged with
Tag:FLAG
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Most of the AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnK446E.αTub84B.T:Zzzz\FLAG hatch successfully.

      AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnK446E.αTub84B.T:Zzzz\FLAG display normal cuticle patterning.

      Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnK446E.αTub84B.T:Zzzz\FLAG fail to survive to adulthood.

      AxnH504L.αTub84B.T:Zzzz\FLAG combined with AxnK446E.αTub84B.T:Zzzz\FLAG can fully rescue AxnS044230.

      Zygotic expression of AxnK446E.αTub84B.T:Zzzz\FLAG does not interfere with development to adulthood. Adults show no visible defects.

      (Kremer et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      AxnH504L.αTub84B.Tag:FLAG/AxnK446E.αTub84B.Tag:FLAG rescues AxnS044230

      (Kremer et al., 2010)
      Partially rescues

      AxnK446E.αTub84B.Tag:FLAG partially rescues AxnS044230

      (Kremer et al., 2010)
      Comments

      Most of the AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnK446E.αTub84B.T:Zzzz\FLAG hatch successfully.

      AxnS044230 mutant (maternal-/zygotic-) embryos expressing maternally provided AxnK446E.αTub84B.T:Zzzz\FLAG display normal cuticle patterning.

      Zygotic AxnS044230 mutants receiving maternally deposited wild-type Axn and expressing AxnK446E.αTub84B.T:Zzzz\FLAG fail to survive to adulthood.

      AxnH504L.αTub84B.T:Zzzz\FLAG combined with AxnK446E.αTub84B.T:Zzzz\FLAG can fully rescue AxnS044230.

      Expression of maternally provided AxnK446E.αTub84B.T:Zzzz\FLAG decreases the hatching rate of embryos to 84.6%.

      (Kremer et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      AxnK446E.αTub84B.T:Zzzz\FLAG
      AxnK446E.αTub84B.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)