FlyBase Allele Report: Dmel\Axn[H504L.αTub84B.Tag:FLAG]

General Information

Symbol

Dmel\Axn^{H504L.αTub84B.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0285787

Feature type

allele

Associated gene

Dmel\Axn

Associated Insertion(s)

Carried in Construct

P{αTub84B(-FRT)Axn.H504L.FLAG}

Key Links

Transgenic product class

missense_variant

(Kremer et al., 2010)

Mutagen

FLPase

Nature of the Allele

Transgenic product class

missense_variant

(Kremer et al., 2010)

Mutagen

FLPase

(Kremer et al., 2010)

Progenitor genotype

Axn^{H504L.FRT.αTub84B.Tag:FLAG}

(Kremer et al., 2010)

Carried in construct

P{αTub84B(-FRT)Axn.H504L.FLAG}

Cytology

Description

Excision of the FRT-w cassette from progenitor Axn^{H504L.FRT.αTub84B.Tag:FLAG} allows the αTub84B promoter to drive expression of an H504L mutant of Axn tagged with N-terminal 6xTag:FLAG.

(Kremer et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

αTub84B

Encoded product / tool

Axn

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | maternal effect, with Axn^S044230

(Kremer et al., 2010)

some die during embryonic stage | maternal effect

(Kremer et al., 2010)

some die during embryonic stage | maternal effect, with Axn^S044230

(Kremer et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

More than half of Axn^S044230 (maternal-/zygotic-) mutant embryos expressing maternally provided Axn^{H504L.αTub84B.T:Zzzz\FLAG} hatch successfully.

Axn^S044230 mutant (maternal-/zygotic-) embryos expressing maternally provided Axn^{H504L.αTub84B.T:Zzzz\FLAG} display normal cuticle patterning.

Zygotic Axn^S044230 mutants receiving maternally deposited wild-type Axn and expressing Axn^{H504L.αTub84B.T:Zzzz\FLAG} survive to adulthood without defects. Subsequently, if the rescued animals are mated with each other, they produce a few larvae and misshapen pupae that subsequently die. This indicates that both sexes of the parents are fertile.

Axn^{H504L.αTub84B.T:Zzzz\FLAG} combined with Axn^{ΔRGS.αTub84B.T:Zzzz\FLAG} can rescue Axn^S044230 mutants to adult viability and normal morphology.

Axn^{H504L.αTub84B.T:Zzzz\FLAG} combined with Axn^{K446E.αTub84B.T:Zzzz\FLAG} can fully rescue Axn^S044230.

Expression of maternally provided Axn^{H504L.αTub84B.T:Zzzz\FLAG} decreases the hatching rate of embryos to 81.9%.

Zygotic expression of Axn^{H504L.αTub84B.T:Zzzz\FLAG} does not interfere with development and survival. Adults show no visible defects.

(Kremer et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Axn^{H504L.αTub84B.Tag:FLAG}/Axn^{K446E.αTub84B.Tag:FLAG} rescues Axn^S044230

(Kremer et al., 2010)

Axn^{ΔRGS.αTub84B.Tag:FLAG}/Axn^{H504L.αTub84B.Tag:FLAG} rescues Axn^S044230

(Kremer et al., 2010)

Partially rescues

Axn^{H504L.αTub84B.Tag:FLAG} partially rescues Axn^S044230

(Kremer et al., 2010)

Comments

More than half of Axn^S044230 (maternal-/zygotic-) mutant embryos expressing maternally provided Axn^{H504L.αTub84B.T:Zzzz\FLAG} hatch successfully.

Axn^S044230 mutant (maternal-/zygotic-) embryos expressing maternally provided Axn^{H504L.αTub84B.T:Zzzz\FLAG} display normal cuticle patterning.

Axn^{H504L.αTub84B.T:Zzzz\FLAG} combined with Axn^{ΔRGS.αTub84B.T:Zzzz\FLAG} can rescue Axn^S044230 mutants to adult viability and normal morphology.

Axn^{H504L.αTub84B.T:Zzzz\FLAG} combined with Axn^{K446E.αTub84B.T:Zzzz\FLAG} can fully rescue Axn^S044230.

(Kremer et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Axn^{H504L.αTub84B.T:Zzzz\FLAG}

Axn^{H504L.αTub84B.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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