FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\AxnΔRGS.αTub84B.Tag:FLAG
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General Information
Symbol
Dmel\AxnΔRGS.αTub84B.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0285526
Feature type
allele
Associated gene
Dmel\Axn
Associated Insertion(s)
Carried in Construct
P{αTub84B(-FRT)Axn.ΔRGS.FLAG}
Also Known As
AxinΔRGS
Key Links
Transgenic product class
inframe_deletion
(Peterson-Nedry et al., 2008)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Peterson-Nedry et al., 2008)
Mutagen
FLPase
(Peterson-Nedry et al., 2008)
Progenitor genotype
AxnΔRGS.FRT.αTub84B.Tag:FLAG
(Peterson-Nedry et al., 2008)
Carried in construct
P{αTub84B(-FRT)Axn.ΔRGS.FLAG}
Cytology
Description

Excision of the FRT-w cassette from progenitor AxnΔRGS.FRT.αTub84B.Tag:FLAG allows the αTub84B promoter to drive expression of an Axn deletion mutant lacking the RGS domain (R53-I172) which is fused with N-terminal 6xTag:FLAG.

(Peterson-Nedry et al., 2008)
Allele components
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
Axn
Tagged with
Tag:FLAG
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      AxnH504L.αTub84B.T:Zzzz\FLAG combined with AxnΔRGS.αTub84B.T:Zzzz\FLAG can rescue AxnS044230 mutants to adult viability and normal morphology.

      AxnL501P.αTub84B.T:Zzzz\FLAG combined with AxnΔRGS.αTub84B.T:Zzzz\FLAG fails to fully rescue AxnS044230 mutants to adult viability. The survival of some of the rescued animals is extended into the pupal stage.

      (Kremer et al., 2010)

      Expression of maternally deposited AxnΔRGS.αTub84B.T:Zzzz\FLAG reduces the hatch rate of embryos to 24% of controls. Examination of the larval cuticles reveal loss of some denticles and occasionally of an entire denticle band.

      The hatch rate of embryos expressing zygotic AxnΔRGS.αTub84B.T:Zzzz\FLAG is not significantly affected. The majority of embryos complete embryonic development. About half of the animals complete development and reach adulthood.

      Heteroallelic combination of AxnΔRGS.αTub84B.T:Zzzz\FLAG and AxnΔArm.αTub84B.T:Zzzz\FLAG rescues AxnS044230 mutants (maternal-/zygotic-) to viability. Cuticle preparations of the rescued embryos reveal a wild-type pattern of denticle band segmentation with only the occasional loss of denticles in row 1. The rescued flies exhibit normal wing formation without any abnormal loss or gain of margin bristles.

      Maternal expression of AxnΔRGS.αTub84B.T:Zzzz\FLAG in AxnS044230 (maternal-/zygotic-) embryos almost completely restores the wild-type pattern of denticle belts, although a few row 1 denticles are frequently missing.

      The majority of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnΔRGS.αTub84B.T:Zzzz\FLAG successfully hatch. However, all of the rescued animals die at later stages.

      (Peterson-Nedry et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      AxnΔRGS.αTub84B.Tag:FLAG/AxnH504L.αTub84B.Tag:FLAG rescues AxnS044230

      (Kremer et al., 2010)
      Partially rescues

      AxnΔRGS.αTub84B.Tag:FLAG/AxnL501P.αTub84B.Tag:FLAG partially rescues AxnS044230

      (Kremer et al., 2010)

      AxnΔRGS.αTub84B.Tag:FLAG partially rescues AxnS044230

      (Peterson-Nedry et al., 2008)

      AxnΔRGS.αTub84B.Tag:FLAG/AxnΔArm.αTub84B.Tag:FLAG partially rescues AxnS044230

      (Peterson-Nedry et al., 2008)
      Comments

      AxnH504L.αTub84B.T:Zzzz\FLAG combined with AxnΔRGS.αTub84B.T:Zzzz\FLAG can rescue AxnS044230 mutants to adult viability and normal morphology.

      AxnL501P.αTub84B.T:Zzzz\FLAG combined with AxnΔRGS.αTub84B.T:Zzzz\FLAG fails to fully rescue AxnS044230 mutants to adult viability. The survival of some of the rescued animals is extended into the pupal stage.

      (Kremer et al., 2010)

      Maternal expression of AxnΔRGS.αTub84B.T:Zzzz\FLAG in AxnS044230 (maternal-/zygotic-) embryos almost completely restores the wild-type pattern of denticle belts, although a few row 1 denticles are frequently missing.

      The majority of AxnS044230 (maternal-/zygotic-) mutant embryos expressing maternally provided AxnΔRGS.αTub84B.T:Zzzz\FLAG successfully hatch. However, all of the rescued animals die at later stages.

      Heteroallelic combination of AxnΔRGS.αTub84B.T:Zzzz\FLAG and AxnΔArm.αTub84B.T:Zzzz\FLAG rescues AxnS044230 mutants (maternal-/zygotic-) to viability. Cuticle preparations of the rescued embryos reveal a wild-type pattern of denticle band segmentation with only the occasional loss of denticles in row 1. The rescued flies exhibit normal wing formation without any abnormal loss or gain of margin bristles.

      (Peterson-Nedry et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      AxnΔRGS.αTub84B.T:Zzzz\FLAG
      AxnΔRGS.αTub84B.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (2)