UASt regulates expression of Sdc lacking its cytoplasmic domain (1-364 aa), tagged at the N-terminus of the extracellular domain (i.e after the signal peptide sequence) with 2x Tag:FLAG and tagged at the C-terminus with GFP.
Scer\GAL4elav.PU/SdcΔC.UAS.Tag:FLAG,GFP rescues Sdc23
Scer\GAL4elav.PU-mediated expression of SdcΔC.Scer\UAS.T:Zzzz\FLAG,T:Avic\GFP fully rescues the Sdc23 ventral midline fascicle cross-over phenotype.