FB2026_02 , released June 18, 2026
Allele: Dmel\mir-989KO
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General Information
Symbol
Dmel\mir-989KO
Species
D. melanogaster
Name
FlyBase ID
FBal0287802
Feature type
allele
Associated gene
Dmel\mir-989
Associated Insertion(s)
TI{TI}mir-989KO
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Kugler et al., 2013)
Mutagen
ends-out gene targeting
(Chen et al., 2014, Kugler et al., 2013)
FLPase
(Chen et al., 2014)
I-SceI
(Chen et al., 2014)
Progenitor genotype
Associated Insertion(s)
TI{TI}mir-989KO
Cytology
Description

The mir-989 hairpin has been deleted. A w+mW.hs marker which is flanked firstly by a pair of loxP sites and secondly (flanking the loxP sites) by a pair of inverted attP sites is present in place of the deleted sequence.

(Chen et al., 2014)
Allele components
Component
Use(s)
Also carries
attP
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos derived from mir-989KO/Df(2R)Exel7130 embryos show a significant decrease in the number of primordial germ cells compared to embryos from control females.

      (Chen et al., 2014)

      Border cell migration is often delayed in the ovaries of homozygous, mir-989KO/Df(2R)50C-38 and mir-989KO/Df(2R)Exel7130 females.

      Wild-type border cells migrate normally when germline cells are homozygous for mir-989KO in mosaic egg chambers. In contrast, migration is delayed when somatic cells including the border cell cluster are homozygous but the germline is wild type. Mixed border cell clusters containing both wild-type and homozygous cells show a delay in migration which is similar in magnitude to the delay seen when the border cell cluster consists entirely of homozygous cells.

      (Kugler et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      mir-989KO is rescued by Scer\GAL4Act.PU/mir-989UAS.cKa

      (Kugler et al., 2013)
      Not rescued by

      mir-989KO is not rescued by mir-989UAS.cKa/Scer\GAL4slbo.2.6

      (Kugler et al., 2013)
      Comments

      Expression of mir-989Scer\UAS.cKa under the control of Scer\GAL4slbo.2.6 fails to rescue the delayed border cell migration seen in mir-989KO females.

      Clonal expression of mir-989Scer\UAS.cKa under the control of Scer\GAL4Act.PU in the border cell cluster rescues the delayed border cell migration seen in mir-989KO females.

      (Kugler et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      miR-989KO
      (Kugler et al., 2013)
      mir-989KO
      (Niu et al., 2019)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)