UASt regulatory sequences drive expression of full-length stumps carrying amino acid substitutions Y515F, Y613F, Y629F, Y844F, Y891F, Y914F, Y930F, Y988F, and Y1009F. The coding sequence is tagged at the N-terminal end with Tag:FLAG.
embryonic/larval tracheal section, with Scer\GAL4unspecified, stumpsunspecified
pericardial cell primordium, with Scer\GAL4unspecified, stumpsunspecified
Scer\GAL4unspecified/stumpsFFF.Y844F.Y891F.Grb2F.UAS.Tag:FLAG fails to rescue stumpsunspecified
Expression of stumpsY844F.Y891F.Grb2F.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4unspecified fails to rescue the tracheal and pericardial precursor cell defects in stumpsunspecified mutant embryos.