FlyBase Allele Report: Dmel\TkR99D[RNAi.UAS]

General Information

Symbol

Dmel\TkR99D^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0287947

Feature type

allele

Associated gene

Dmel\TkR99D

Associated Insertion(s)

Carried in Construct

P{UAS-TkR99D.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Ignell et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ignell et al., 2009)

Mutagen

in vitro construct

(Ignell et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-TkR99D.RNAi}

Cytology

Description

UAS regulates expression of an RNAi construct targeting TkR99D.

(Ignell et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

TkR99D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology, with Scer\GAL4^{Orco.2.642.Hsim\VP22}

(Ignell et al., 2009)

abnormal smell perception, with Scer\GAL4^{Orco.2.642.Hsim\VP22}

(Ignell et al., 2009)

Phenotype Manifest In

antennal lobe glomerulus DM1 | chemical conditional

(Ignell et al., 2009)

antennal lobe glomerulus DM2 | chemical conditional

(Ignell et al., 2009)

antennal lobe glomerulus DM5 | chemical conditional

(Ignell et al., 2009)

antennal lobe glomerulus VM2 | chemical conditional

(Ignell et al., 2009)

synaptic vesicle, with Scer\GAL4^{Orco.2.642.Hsim\VP22}

(Ignell et al., 2009)

Detailed Description

Statement

Reference

Scer\GAL4^{Orco.2.642.T:Hsim\VP22}-mediated expression of TkR99D^{dsRNA.Scer\UAS} results in a larger increase in synaptic vesicle release following electrical stimulation of the olfactory nerve, compared to controls, and abolished the normal sensitivity of the olfactory nerve to Tk application.

Scer\GAL4^{Orco.2.642.T:Hsim\VP22}-mediated expression of TkR99D^{dsRNA.Scer\UAS} significantly increases olfactory responses in the DM1 and DL5 glomeruli following exposure to a high concentration of ethyl-3-hydroxybutyrate.

Scer\GAL4^{Orco.2.642.T:Hsim\VP22}-mediated expression of TkR99D^{dsRNA.Scer\UAS} significantly increases olfactory responses in the DM1 and DM2 and VM2 glomeruli following exposure to a high concentration of methyl hexanoate.

Scer\GAL4^{Orco.2.642.T:Hsim\VP22}-mediated expression of TkR99D^{dsRNA.Scer\UAS} results in an increase in behavioural response index at high concentrations of ethyl-3-hydroxybutyrate and methyl hexanoate.

(Ignell et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

TkR99D^RNAi.UAS

TkR99D^{dsRNA.Scer\UAS}

TkR99D^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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