FlyBase Allele Report: Mmus\Wld[S-dead.UAS]

General Information

Symbol

Mmus\Wld^S-dead.UAS

Species

M. musculus

Name

FlyBase ID

FBal0288267

Feature type

allele

Associated gene

Mmus\Wld

Associated Insertion(s)

Carried in Construct

P{UAS-wld.S-dead}

Also Known As

UAS-Wld^S-dead

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Avery et al., 2009)

Progenitor genotype

Mmus\Wld^S.UAS

(Avery et al., 2009)

Carried in construct

P{UAS-wld.S-dead}

Cytology

Description

UASt regulatory sequences drive expression of a mutant form of Mmus\Wld in which the histidine at amino 24 has been replaced by alanine.

(Avery et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Wld

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Or22a.8197

(Avery et al., 2009)

abnormal neuroanatomy | conditional, with Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2014)

Phenotype Manifest In

olfactory receptor neuron, with Scer\GAL4^Or22a.8197

(Avery et al., 2009)

wing vein L1 | conditional, with Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2014)

Detailed Description

Statement

Reference

Expression of one copy of Mmus\wld^{S-dead.Scer\UAS} under the control of Scer\GAL4^VGlut-OK371 has a moderate protective effect on L1 vein neurons following axotomy. Fragmentation of axons occurs more slowly than in wild type.

(Neukomm et al., 2014)

Expression of Mmus\wld^{S-dead.Scer\UAS} under the control of Scer\GAL4^Or22a.8197 has very little protective effect on olfactory receptor neurons following axotomy: 92% of olfactory receptor neuron axons have undergone Wallerian degeneration within 15 days of injury.

(Avery et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Mmus\Wld^S-dead.UAS/Scer\GAL4^ppk.PH is a non-enhancer of abnormal neuroanatomy | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP

(Ji et al., 2022)

Mmus\Wld^S-dead.UAS/Scer\GAL4^ppk.PH is a non-enhancer of decreased size | larval stage phenotype of Nmnat^U6:3.gRNA, Spyo\Cas9^ppk.PP