FlyBase Allele Report: Mmus\Wld[S-dead.UAS.Tag:MYC]

General Information

Symbol

Mmus\Wld^{S-dead.UAS.Tag:MYC}

Species

M. musculus

Name

FlyBase ID

FBal0288268

Feature type

allele

Associated gene

Mmus\Wld

Associated Insertion(s)

Carried in Construct

P{UAS-wld.S-dead.MYC}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Avery et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-wld.S-dead.MYC}

Cytology

Description

UASt regulatory sequences drive expression of a mutated form of the fusion protein associated with the mouse 'Wld[S]' mutation. The 'Wld[S]' fusion protein is composed of the N-terminal 70 amino acids of Mmus\Ube4b, a unique 18 amino acid domain generated during the fusion event and the full-length sequence of Mmus\Nmnat1. In this transgene, an H24A amino acid replacement has been introduced into the fusion protein sequence (this mutation largely abolishes Mmus\Nmnat1 enzymatic activity). The coding sequence is tagged at the C-terminal end with 6xTag:MYC.

(Avery et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mmus\Wld

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Mmus\Wld^{S-dead.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 is a suppressor of abnormal neuroanatomy | adult stage phenotype of Nmnat^JF03337, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Scer\GAL4^ey-OK107/Mmus\Wld^{S-dead.UAS.Tag:MYC} is a suppressor of abnormal neuroanatomy | somatic clone | adult stage | progressive phenotype of bsk^flp147E

(Rallis et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

Mmus\Wld^{S-dead.UAS.Tag:MYC}, Scer\GAL4^ey-OK107 is a suppressor of alpha/beta Kenyon cell | adult stage phenotype of Nmnat^JF03337, Scer\GAL4^ey-OK107

(Rallis et al., 2013)

Scer\GAL4^ey-OK107/Mmus\Wld^{S-dead.UAS.Tag:MYC} is a suppressor of alpha/beta Kenyon cell phenotype of bsk^flp147E

(Rallis et al., 2013)

Scer\GAL4^ey-OK107/Mmus\Wld^{S-dead.UAS.Tag:MYC} is a suppressor of gamma Kenyon cell phenotype of bsk^flp147E

(Rallis et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\wld^{S-dead.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey-OK107 suppresses the axon degeneration seen in bsk^flp147E mutant mushroom body neuron clones at 28 days. The number of enlarged and supernumerary axonal protrusions seen on bsk^flp147E mutant axon shafts is also significantly reduced.

Expression of Mmus\wld^{S-dead.Scer\UAS.T:Hsap\MYC} suppresses the mushroom body degeneration seen in 7 day old flies when Nmnat^JF03337 is expressed under the control of Scer\GAL4^ey-OK107.

(Rallis et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mmus\Wld^{S-dead.Scer\UAS.T:Hsap\MYC}

Mmus\Wld^{S-dead.UAS.Tag:MYC}

Mmus\wld^{S-dead.Scer\UAS.T:Hsap\MYC}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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