FlyBase Allele Report: Ecol\lexA[GMR23E10]

General Information

Symbol

Ecol\lexA^GMR23E10

Species

E. coli

Name

FlyBase ID

FBal0289160

Feature type

allele

Associated gene

Ecol\lexA

Associated Insertion(s)

Carried in Construct

P{GMR23E10-lexA}

Also Known As

R23E10-lexA, 23E10-LexA

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2013.10.9)

Progenitor genotype

Carried in construct

P{GMR23E10-lexA}

Cytology

Description

A 771 base pair fragment from the flanking non-coding or intronic region of AlstR is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Pfeiffer et al., 2013.10.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR23E10

Encoded product / tool

lexA::p65

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage P7 -- pharate adult stage P15

adult lateral fan-shaped body tangential-protocerebrum neuron ExFl2

(Chakravarti Dilley et al., 2020)

adult stage

adult neuron | subset of adult dorsal fan-shaped body

(Lei et al., 2022)

adult neuron | subset of adult brain

(Lei et al., 2022)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The silencing of Ecol\lexA^GMR23E10-expressing neurons through Hsap\KCNJ2^lexAop.EGFP expression (Ecol\lexA^GMR23E10>Hsap\KCNJ2^lexAop.EGFP) does not affect sleep duration, as compared to controls.

(Toda et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ecol\lexA^GMR23E10/TrpA1^lexAop.cUa is a suppressor | partially of decreased sleep | adult stage phenotype of IM33^HMS04250, Scer\GAL4^repo

(Xu et al., 2023)

Ecol\lexA^GMR23E10/TrpA1^lexAop.cUa is a suppressor | partially of abnormal sleep | adult stage phenotype of IM33^HMS04250, Scer\GAL4^repo

(Xu et al., 2023)

NOT Suppressor of

Statement

Reference

Ecol\lexA^GMR23E10/TrpA1^lexAop.cUa is a non-suppressor of short lived | male phenotype of IM33^HMS04250, Scer\GAL4^repo

(Xu et al., 2023)

Hsap\KCNJ2^lexAop.EGFP/Ecol\lexA^GMR23E10 is a non-suppressor of abnormal sleep | RU486 conditional phenotype of Scer\GAL4^{elav.Switch.PO}, nur^{UAS.Tag:FLAG,Tag:HA}

(Toda et al., 2019)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The increased sleep induced by conditional adulthood-only expression of nur^{UAS.Tag:FLAG,Tag:HA} under the combined control of Scer\GAL4^{elav.Switch.PO} and RU486 feeding is not suppressed by silencing Ecol\lexA^GMR23E10-expressing neurons through Hsap\KCNJ2^lexAop.EGFP expression (Ecol\lexA^GMR23E10>Hsap\KCNJ2^lexAop.EGFP).

(Toda et al., 2019)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

94681

w¹¹¹⁸; P{GMR23E10-lexA}JK22C

52693

w¹¹¹⁸; P{GMR23E10-lexA}attP40

Korea

13916

P{GMR23E10-lexA}JK73A

Notes on Origin

Discoverer

Comments

Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.

(Pfeiffer et al., 2013.10.9)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ecol\lexA^GMR23E10

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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