A 3387 base pair fragment from the flanking non-coding or intronic region of CG30106 is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
EcollexAGMR24E12 drives expression in 17-19 mushroom body extrinsic neurons, including dopaminergic PAM neurons 13 and 14 and mushroom body output neuron 5, that innervate mushroom body beta' lobe slice 1. Expression in dopaminergic PAM neurons 13 and 14 overlaps with that of ScerGAL4VT028152, ScerGAL4amn-NP2397, ScerGAL4amn-NP7177, ScerGAL4GMR66A09, ScerGAL4VT040039, ScerGAL4GMR94F11, ScerGAL4GMR58E02 and ScerGAL4VT028152 while expression in mushroom body output neuron 5 overlaps with that of ScerGAL4GMR76B09, ScerGAL4GMR18H09, and ScerGAL4VT026001.
Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.