FlyBase Allele Report: Ecol\lexA[GMR75E05]

General Information

Symbol

Ecol\lexA^GMR75E05

Species

E. coli

Name

FlyBase ID

FBal0290089

Feature type

allele

Associated gene

Ecol\lexA

Associated Insertion(s)

Carried in Construct

P{GMR75E05-lexA}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2013.10.9)

Progenitor genotype

Carried in construct

P{GMR75E05-lexA}

Cytology

Description

A 3284 base pair fragment from the flanking non-coding or intronic region of ab is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Pfeiffer et al., 2013.10.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR75E05

Encoded product / tool

lexA::p65

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Ecol\lexA^GMR75E05/drpr^RNAi.lexAop is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of αSnap^KK107910, Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

(Coutinho-Budd et al., 2017)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Ecol\lexA^GMR75E05/drpr^RNAi.lexAop is a non-suppressor of embryonic/larval astrocyte-like glial cell | third instar larval stage phenotype of αSnap^KK107910, Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

(Coutinho-Budd et al., 2017)

Ecol\lexA^GMR75E05/drpr^RNAi.lexAop is a non-suppressor of larval ventral nerve cord | third instar larval stage phenotype of αSnap^KK107910, Hsim\VP16^AD.nrv2, Scer\GAL4^{DBD.wrapper.932i}

(Coutinho-Budd et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

spz3^{13xlexAop.EGFP}/Ecol\lexA^GMR75E05 fails to rescue spz3^KK106772

(Coutinho-Budd et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

52710

w¹¹¹⁸; P{GMR75E05-lexA}attP40/CyO

Notes on Origin

Discoverer

Comments

Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.

(Pfeiffer et al., 2013.10.9)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ecol\lexA^GMR75E05

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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