FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Sas-4ΔT.GFP
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General Information
Symbol
Dmel\Sas-4ΔT.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0290358
Feature type
allele
Associated gene
Dmel\Sas-4
Associated Insertion(s)
Carried in Construct
P{Sas-4-GFP.ΔT}
Key Links
Transgenic product class
missense_variant
(Gopalakrishnan et al., 2012)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Gopalakrishnan et al., 2012)
Mutagen
in vitro construct
(Gopalakrishnan et al., 2012)
Progenitor genotype
Carried in construct
P{Sas-4-GFP.ΔT}
Cytology
Description

The Sas-4 promoter drives expression of full-length Sas-4 with mutations in the two amino acid residues essential for tubulin binding (amino acid replacements R114E and R115E). The protein is tagged at the C-terminal end with GFP.

(Gopalakrishnan et al., 2012)
Allele components
Component
Use(s)
Regulatory region(s)
Sas-4
Encoded product / tool
Sas-4
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The sperm axonemes of Sas-4s2214 males rescued with Sas-4ΔT.T:Avic\GFP are abnormal, but the number of centrosomes is normal. Interphase spermatocyte centrosomes show premature microtubule nucleation and meiotic centrosomes have massive microtubule asters, which can fill a significant fraction of the cell. Over 95% of round spermatids show normal morphology, suggesting that meiotic cell division can conclude normally.

      (Gopalakrishnan et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Sas-4ΔT.GFP partially rescues Sas-4s2214

      (Gopalakrishnan et al., 2012)
      Comments

      Sas-4ΔT.T:Avic\GFP fails to fully rescue the Sas-4s2214 phenotype of uncoordination: the flies stand but can barely walk.

      (Gopalakrishnan et al., 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Sas-4ΔT.GFP
      Sas-4ΔT.T:Avic\GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)