A Nrx-1 promoter drives expression of ewg. The 5' end of ewg is derived from cDNA sequence, while the 3' end is derived from genomic DNA (intron sequences from intron 4 onwards are present). A Tag:VSV-G tag and a Tag:HA tag have been added at the end of the open reading frames resulting from intron 6 spliced and unspliced mRNAs respectively.
ewgtcgR.Nrx-1.T:Ivir\HA1,T:VSV\G fully rescues the lethality of Df(1)ewgΔ animals.